LOCUS       sequence-73183-e         747 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of the
            blue fluorescent protein AmCyan and the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 747)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 747)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Smurf
FEATURES             Location/Qualifiers
     source          1..747
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(35..68)
                     /label=lox5171
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTGTAC) (Shaw et al., 2021). lox5171 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     CDS             complement(90..143)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(165..221)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 attttgtaat ccagaggttg attgtcgagt cgacataact tcgtatagta cacattatac
       61 gaagttatga attctgatca cacgctagct gggccgggat tttcctccac gtccccgcat
      121 gttagtagac ttcccctgcc ctcgccggag ccggatcctc tagaaggccc gggattctcc
      181 tccacgtcac ctgcttgttt gagtagtgag aagtttgttg ctccagatcc cttgtacacg
      241 aagggcacca cggaggtgat gtgggccacg gcgtgctcgg tcagctgcac gctgttgccg
      301 cccttgtcca ggtcggttct ggcgatgcgg tgctccacca cgtggttggg gggcatggtc
      361 acgggcttct tggtcttgta ggaggtgtgg aactggcatc tgtagttgcc gccgccctgc
      421 agcatcagga aggcggtcac gtcgcccttc aagatgccgt cgcacacggt catcttctcg
      481 aaggaggggt cccagccggt ggtcttcttg gccatcacgg ggccgtcggc ggggaagttc
      541 acgccgtgga aggtggactt gtgctcgaag cagttgccct tcaggctgat ctcccagctg
      601 gcggtggcca cgccgccgtc ctcgtaggtg aaggttctct cgtaggacat gccgtcgggg
      661 aaggcctgct tgaagtagtc gggcatgctg gtggggtagg cggtgaagca gcggttgccg
      721 tacatgaaca cggtggacag gatgtcg
//
