LOCUS       sequence-73184-e         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  3rd generation Lentiviral vector with bi-cistronic expression of 
            dtomato and the gene of interest..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  TITLE     pUltra series
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pUltra-Chili
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(68..121)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(143..199)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(292..315)
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(490..509)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 aattttgtaa tccagaggtt gattgtcgag tcgacgactc cggaacgaat tctgatcaca
       61 cgctagctgg gccgggattt tcctccacgt ccccgcatgt tagtagactt cccctgccct
      121 cgccggagcc ggatcctcta gaaggcccgg gattctcctc cacgtcacct gcttgtttga
      181 gtagtgagaa gtttgttgct ccagatccct tgtacagctc gtccatgccg tacaggaaca
      241 ggtggtggcg gccctcggag cgctcgtact gttccacgat ggtgtagtcc tcgttgtggg
      301 aggtgatgtc cagcttggtg tccacgtagt agtagccggg cagttgcacg ggcttcttgg
      361 ccatgtagat ggtcttgaac tccaccaggt agtggccgcc gtccttcagc ttcagggcct
      421 ggtggatctc gcccttcagc acgccgtcgc gggggtacag gcgctcggtg gaggcctccc
      481 agcccatggt cttcttctgc attacggggc cgtcgggggg gaagttggtg ccgcgcatct
      541 tcaccttgta gatcagcgtg ccgtcctgca gggaggagtc ctgggtcacg gtcaccagac
      601 cgccgtcctc gaagttcatc acgcgctccc acttgaagcc ctcggggaag gacagcttct
      661 tgtaatcggg gatgtcggcg gggtgcttca cgtacgcctt ggagccgtac atgaactggg
      721 gggacaggat
//
