LOCUS       sequence-73187-e         638 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  U6-driven sgRNA expressing cassette on a gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 638)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC155-pCR8-sgExpression
FEATURES             Location/Qualifiers
     source          1..638
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    70..94
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(190..265)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(284..532)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(342..361)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(512..532)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     protein_bind    complement(543..567)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 atgattttat tttgactgat agtgacctgt tcgttgcaac aaattgatga gcaatgcttt
       61 tttataatgc caactttgta caaaaaagca ggctccgcct ctagagccat ttgtctgcag
      121 aattggcgca cgcgctaaaa acggactagc cttattttaa cttgctattt ctagctctaa
      181 aacaaaaaag caccgactcg gtgccacttt ttcaagttga taacggacta gccttatttt
      241 aacttgctat ttctagctct aaaacaggtc ttctcgaaga cccggtgttt cgtcctttcc
      301 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      361 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      421 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattccaatt atctctctaa
      481 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcacatgtgg
      541 cgacccagct ttcttgtaca aagttggcat tataaaaaat aattgctcat caatttgttg
      601 caacgaacag gtcactatca gtcaaaataa aatcatta
//
