LOCUS       sequence-73198-e         803 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian U6-driven sgRNA (SPm) targeting GTCCCCTCCACCCCACAGTG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 803)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 803)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: M-SP-sgRNA
FEATURES             Location/Qualifiers
     source          1..803
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    92..116
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_RNA        complement(128..203)
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        complement(224..472)
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     complement(282..301)
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(452..472)
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     complement(611..630)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(612..630)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(637..654)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(637..653)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(646..668)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 ctatagaata ctcaagctat gcatcaagct tggtaccgag ctcggatcca ctagtaacgg
       61 ccgccagtgt gctggaattc gccctttaat gccaactttg tacaagaaag ctgggtctag
      121 aaaaaaagca ccgactcggt gccacttttt caagttgata acggactagc cttattttaa
      181 cttgctattt ctagctctaa aaccactgtg gggtggaggg gacggtgttt cgtcctttcc
      241 acaagatata taaagccaag aaatcgaaat actttcaagt tacggtaagc atatgatagt
      301 ccattttaaa acataatttt aaaactgcaa actacccaag aaattattac tttctacgtc
      361 acgtattttg tactaatatc tttgtgttta cagtcaaatt aattctaatt atctctctaa
      421 cagccttgta tcgtatatgc aaatatgaag gaatcatggg aaataggccc tcttcctgcc
      481 cgaccttggt accggatcca gtcgactgaa ttggttcctt taaagcctgc ttttttgtac
      541 aaagggcgaa ttctgcagat atccatcaca ctggcggccg ctcgagcatg catctagagg
      601 gcccaattcg ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg
      661 tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc
      721 cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct
      781 atacgtacgg cagtttaagg ttt
//
