LOCUS       sequence-73204-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  full length coding sequence for zebrafish lin28 gene in gateway 
            middle entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Moore JC, Sheppard S, Shestopalov IA, Chen JK, Lawson N
  TITLE     Post-transcriptional mechanisms contribute to Etv2 repression during
            vascular development.
  JOURNAL   Dev Biol. 2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028.
  PUBMED    24036310
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Biol. 
            2013 Sep 11. pii: S0012-1606(13)00470-3. doi: 
            10.1016/j.ydbio.2013.08.028."
COMMENT     Sequence Label: 1132 pME-Lin28
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    2..101
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(720..819)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(836..855)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(837..855)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(860..876)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(860..876)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 gcccaatatg attttatttt gactgatagt gacctgttcg ttgcaacaaa ttgatgagca
       61 atgctttttt ataatgccaa ctttgtacaa aaaagcaggc tgcgccacca tgcccccggc
      121 aaatccgcat ctcaatcaca caggtggatg tacaaagaca gaggaggaag aagccgcgag
      181 ctccgaggaa gactcaggct cttttcacgg ctctggggtc tgcaaatggt ttaacgtgcg
      241 gatgggcttc ggatttctgt ccatgaccca ccgggagggc atctgtctgg actcacccgt
      301 ggatgtcttc gtccatcaga gcaagcttca tatggagggt tttcgcagtc tgaaggaagg
      361 agaagcagtg gaattcacct ttaaaagatc ctctaagggt ctggagtcct tacaagtgac
      421 ggggcctgga ggggcaccat gtgtgggcag cgagaagaaa cctaagggta cccaaaagag
      481 gcggtcaaaa ggagatcgat gcttcaactg tggaggaccc aatcatcatg ccaaagaatg
      541 ccagctgcca cctcagccga aaaagtgcca tttttgccaa agcatctccc acatggtggc
      601 caactgccca atcaaagcac agcagttgtc accaggatct cagggcaaat cgacaacctc
      661 aacaggagaa gaggaggaca tgagccacac tcccttactg ccagagagca ctgattagga
      721 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
      781 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg atatccccta
      841 tagtgagtcg tattacatgg tcatagctgt ttcctggcag ctctggcccg tgtctcaaaa
      901 tctctgatgt tacattgcac
//
