LOCUS       sequence-73205-e        7153 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with C8ORF46 (Ple251) promoter driving expression of 
            iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7153)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7153)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1977
FEATURES             Location/Qualifiers
     source          1..7153
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2710..2842
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2825..2844
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3923..4044
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3934..3953)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3988..4007
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4214..4343
                     /label=AAV2 ITR
     primer_bind     complement(4362..4379)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4362..4378)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4371..4393)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4520..4975
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4607..4626)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4817..4838
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5001..5105
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5106..5174,5175..5966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5324..5343)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6137..6725
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6626..6645
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7013..7034
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7049..7054,7055..7072,7073..7079)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7087..7103
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7092..7114
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7111..7127
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7111..7127
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg ccaataaatg ataagcacaa
      241 agtgatcaaa actacactcc ttgtattgtg agattaacct aaccacaata taatctttca
      301 aaattagtac ctcttttcct taaatacgtc ttgtatttcc aactcttctg ttattccacc
      361 tgagaggtat acaggtgtgt gggcctcaaa taagaaggaa aaaaaaagag aagaggaaag
      421 aaaataggtc ctttctggaa ggcagacagc gttggcaagc tagcagcaga ttcagttctg
      481 gaaaataaag cccatgtgct ttgaggtggt tggggagtca tttgctctta gatctccatt
      541 tcacctgcct cacagctgtc ctgctgcatg cttcatcaac attagggcag gcatgtttgt
      601 gccagcttct tttcatatgt caatcaaggg acatttgttc agttacttac tacatacatt
      661 gcatgagcta aatgcaaccc agcctggatg tccccacccc atcctgattc ccatcctgtg
      721 ttctgagatg cctgttttca ctcataaaaa ccgaaatgca acaggacttt aaactgttca
      781 gaagagaact ttgtgttaag aaaatgcaac ttcttctgct tggcctacac caaagtacaa
      841 agagattggt gtgaaatggc tttctcttgg gaggaatgga gctaagtgag acatgttcag
      901 ttgcttccca gggacatgag aaggagttat tcttctcttt gcagaatgct ggtggagaat
      961 cactgtatag ggcagtaaaa tccttcctaa tgaagtattt ggggcttacc tttgctcaag
     1021 agggaaagaa ggacatctct ttctccaagt tgtggccaca ggtctcacaa tgtcaaaata
     1081 tctcctaagg ccttaactgt cttatgcttt gatataaagg aaaaaaggaa atgccagtta
     1141 aataaggcat aggccaagag gtaccttggg agactctctc tgggggtccc ggtgtcctgt
     1201 ctgtctgcag atgcacacac cctgggcgct cctggggcgg gccctggaca gagggatgca
     1261 gcgcagatcc atcctggctg acaccctgca ctgaagactc ctaacaatgg cccggggcca
     1321 gccgcgctca cccaagtgca aacatcgctc tagtctctgc ccacaaaggc cagatggaaa
     1381 ctccccctca tttgttggct cagatctgac agagcaaaag ccttttatcc cagaggaaag
     1441 agccggttct gagcggcaac cccgcccccg agcacacaca aagtcaaaaa gacgtttgtc
     1501 cgaagggcct gtcacaggga agacgttctt ttatttattt cgtttttaaa taattttctc
     1561 ttcccgccaa aatgtccgta gtagtagcag cagcagcagg tccacgggga gcctgccctc
     1621 ggtcctgctt gtcacatccc taatcgcgtc ccatctggtg tctggcgtgc acaaaggccg
     1681 cccgttccgg cgggttgtgc atttatgcag cgcagcggcc gccccgcacc aggccgacgc
     1741 gacacacttc tggcaggtgc gggccagggc catccgcagg ctacatgccc gccgcgccct
     1801 cagcctctcc agggctcggg ccacacaaaa ggcgccgggt gctgcgggct tgggccccct
     1861 cccgctccgg gctggcaccg ggactgacgg ctgatgtggc gggacagtat ataatctgta
     1921 acctgtatat gtagcataat agggtttata taatatatta ggatatatag ggtaatacaa
     1981 atataacttt ctataatttg ctccttgaac atggggcact gcctgttgcc atgtctctgt
     2041 gagcacctaa gggttatacg cggaggtacc aaacacagga cccttgagaa atgtcagcct
     2101 tgtcatgtaa tggcatgagg ggaaaacact ggagaaacag tccttttgtt taaaatagaa
     2161 acttctaaga gagccaagtt agcacatgac aaattactga atatcccgtt ctcctggagg
     2221 ggagaaaaaa aaaaaagccc cttcatttct gaaatgagaa agggaattgc tggtaccaaa
     2281 attcacgttt cctttttggt ggaaattctc cagccaagct tggcgctaac agagtaggcg
     2341 atttcacttc gtcgtcacct cacaaaaggc ttccaaattc actgcggcag acaccgcaaa
     2401 ataaaaatgt taaatacatc ctagctggga ggggattgaa gcctccaggc tcaatcaaag
     2461 gtttgcaata ataggattca tttaaaaaaa aaaaaaaaaa aggacagatg ggtttagaaa
     2521 atgttgcagt gaagactgga acgagatgag ctcatagcac cagcctgtct tttgtggacc
     2581 cgcacaatga tccctgagcc agactggatt aggatgcctc gcgactaggg gtccagagac
     2641 agaggcctcc agttcccagg cacttcggga agaggggcgc gccgaagttg gtcgtgaggc
     2701 actgggcagg taagtatcaa ggttacaaga caggtttaag gagaccaata gaaactgggc
     2761 ttgtcgagac agagaagact cttgcgtttc tgataggcac ctattggtct tactgacatc
     2821 cactttgcct ttctctccac aggtgtccac tcccaggcgg ccgccaccat gtccaacctg
     2881 ctgactgtgc accaaaacct gcctgccctc cctgtggatg ccacctctga tgaagtcagg
     2941 aagaacctga tggacatgtt cagggacagg caggccttct ctgaacacac ctggaagatg
     3001 ctcctgtctg tgtgcagatc ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc
     3061 cctgctgaac ctgaggatgt gagggactac ctcctgtacc tgcaagccag aggcctggct
     3121 gtgaagacca tccaacagca cctgggccag ctcaacatgc tgcacaggag atctggcctg
     3181 cctcgccctt ctgactccaa tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat
     3241 gtggatgctg gggagagagc caagcaggcc ctggcctttg aacgcactga ctttgaccaa
     3301 gtcagatccc tgatggagaa ctctgacaga tgccaggaca tcaggaacct ggccttcctg
     3361 ggcattgcct acaacaccct gctgcgcatt gccgaaattg ccagaatcag agtgaaggac
     3421 atctcccgca ccgatggtgg gagaatgctg atccacattg gcaggaccaa gaccctggtg
     3481 tccacagctg gtgtggagaa ggccctgtcc ctgggggtta ccaagctggt ggagagatgg
     3541 atctctgtgt ctggtgtggc tgatgacccc aacaactacc tgttctgccg ggtcagaaag
     3601 aatggtgtgg ctgccccttc tgccacctcc caactgtcca cccgggccct ggaagggatc
     3661 tttgaggcca cccaccgcct gatctatggt gccaaggatg actctgggca gagatacctg
     3721 gcctggtctg gccactctgc cagagtgggt gctgccaggg acatggccag ggctggtgtg
     3781 tccatccctg aaatcatgca ggctggtggc tggaccaatg tgaacatagt gatgaactac
     3841 atcagaaacc tggactctga gactggggcc atggtgaggc tgctcgagga tggggactga
     3901 gcggccgcga tcgcagacat gataagatac attgatgagt ttggacaaac cacaactaga
     3961 atgcagtgaa aaaaatgctt tatttgtgaa atttgtgatg ctattgcttt atttgtaacc
     4021 attataagct gcaataaaca agttaacaac aacaattgca ttcattttat gtttcaggtt
     4081 cagggggaga tgtgggaggt tttttaaagc aagtaaaacc tctacaaatg tggtaggtcg
     4141 aaattcccga taaggatctt cctagagcat ggctacgtag ataagtagca tggcgggtta
     4201 atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct gcgcgctcgc
     4261 tcgctcactg aggccgggcg accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc
     4321 tcagtgagcg agcgagcgcg cagccttaat taacctaatt cactggccgt cgttttacaa
     4381 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     4441 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     4501 agcctgaatg gcgaatggga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg
     4561 gttacgcgca gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc
     4621 ttcccttcct ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc
     4681 cctttagggt tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt
     4741 gatggttcac gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag
     4801 tccacgttct ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg
     4861 gtctattctt ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag
     4921 ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttaggtg
     4981 gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa
     5041 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
     5101 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
     5161 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
     5221 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
     5281 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
     5341 tatcccgtat tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
     5401 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
     5461 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
     5521 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
     5581 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
     5641 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
     5701 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
     5761 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
     5821 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
     5881 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
     5941 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
     6001 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
     6061 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
     6121 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
     6181 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
     6241 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta gtgtagccgt
     6301 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
     6361 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
     6421 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
     6481 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
     6541 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
     6601 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
     6661 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
     6721 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
     6781 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
     6841 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
     6901 cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     6961 gctggcacga caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga
     7021 gttagctcac tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt
     7081 gtggaattgt gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca
     7141 gatttaatta agg
//
