LOCUS       sequence-73212-e        7170 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with PITX3 (Ple253) promoter driving expression of iCre.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7170)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7170)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1983
FEATURES             Location/Qualifiers
     source          1..7170
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2727..2859
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2842..2861
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3940..4061
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3951..3970)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4005..4024
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4231..4360
                     /label=AAV2 ITR
     primer_bind     complement(4379..4396)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4379..4395)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4388..4410)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4537..4992
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4624..4643)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4834..4855
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5018..5122
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5123..5191,5192..5983)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5341..5360)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6154..6742
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6643..6662
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7030..7051
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7066..7071,7072..7089,7090..7096)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7104..7120
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7109..7131
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7128..7144
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7128..7144
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac ctggccacga tgctctagtg taaagcaccc
      241 atcagatatc agacgtgaag aacccctcag taacttccta cacctgcaca ttgggcttct
      301 ggtcggtggg tccagctgag gatctagcct ctatcaccca aagagagtca gggaaagagg
      361 atataccatc atagcacctc tttccacatt acctcaaagc cattttcctc ttcatttgaa
      421 aactgagagt tggtgtcctg ggcctgtctc agattgagag gtcctggaaa gccgattgga
      481 aaatcctcta agtgggccaa aaatttctgc tcgccaatat ctacttctac atacctccag
      541 aaattcatca ttctaaaagc atgtgattgt gcaatagaga tggggaggag agagatgaca
      601 attccctagt ccctcctaac cactaatcat catcttacca ggcagaacat cttcctcata
      661 gatttcagtg tgacatttat tgagattcta cattatgcct gggaataaca gacatggtcc
      721 ttaccctcct aaattttatt atcatagcag acaacacata agctgatccc ttcaatatgg
      781 tgggtggtgt aatagaaggt atacactgag ggtcatgaga gcaaggggct aaactgagag
      841 ttcagaaaag gcttcccaga gaaaataaga cttgagtgga acattggagg agtaggaatt
      901 gacaatataa agatgggtta ctttcacagg aagggccaga ggtgtgcagg acactactag
      961 gaaattggag atgaagacaa ggtcaggtcc tgaaggtctt gtatgccagg ctagggagct
     1021 tgacctttat cctaagggca atgggagtca ctgaacagtc aagacatgac tgctcagatt
     1081 tgccttaggg gcatattgtg tgactgggct taggctcttt tttttttttt tgagacagag
     1141 tcttgctctg tcgcccagtc tggagtgcag tggcgcgatc tcagctcact gcaagctctg
     1201 cctcctgggt tcacgccatt cttcttcctc agcctcccca gtagctgaga ctacaggcac
     1261 ccgccaccaa gcccggctaa ttttttgtat tttttagtag agatggggtt tcaccgtgtt
     1321 agccagggtg gtctcaatct cctgaccttg tgatccgccc gcctcggcct cccaaagtgc
     1381 tgggattaca ggcatgagcc acagtgtctg gcctgggctt aggctcttcg gagcacccaa
     1441 gcaacatagc ctaactggga gaagagaagg agcctgtttc ctggaaattg aggcctaagt
     1501 cagagagggc cgaagtcaca gtctgccagg atgttccttg gtcatctgtc ctgtcccttg
     1561 caaattccta accagcaggc accaaggcca caccttgccc acaggactta acctggtacc
     1621 accagcgaga aaccagccct gctgaaggag ggagagcaca gcctaagcaa gacaagggta
     1681 cagactgaaa ggcaatgcct ttggagtgcc cagcccaagc cctgagatcc agcaggaacc
     1741 tcctcttaat tgttttgcgc ggggagggag atgatagtta ctcgtccaag ttcactcagt
     1801 gatggtgcag ttcgtggcag cgttggaata ctactttggt ttgtatcatt ccaaaccctg
     1861 ctgtctccac gaagtcacct tccttggaga agaaggggga aatgaagaag aggaaagata
     1921 tgaggaaaga ggagcagaga gaaagagaaa tgagtctttt aaagtggcag aggaaggaga
     1981 atggtaaaca gcaggagcga agcggctgag gagaaagaag aggaaagaaa ggcgagacgt
     2041 gggaggattg gaacagagac aaaagggagg agagacggac agcgacaagt ggagaaaatc
     2101 ggcgaaactt gagtggcaga gaagtctgag cgctgagacc cggcggcccc gtgcgccttc
     2161 ccacctggcg ccgatccact ttcctcgggg tagcggccca acccacttcg ctgccagccg
     2221 atccctttta cccgtggcta ccgggaccac tctactctcg cccacttggc tctgcctaag
     2281 cgtcctagcc ggagcgcggt ctctgccacg tggggagggg cgcggccgag ttgctgaaga
     2341 gcgcttctga ttggccagag ggcggggttc ttggcgtctc gccggccaga cccctccctc
     2401 aaaggcgggg cctggagatc cacagctgga aagggcggag ccccagcagg acagctggaa
     2461 aggggcgggg cctgacgcgc gcggctcgcc gcggcgggct gggggcgccc tggtctgcca
     2521 taaagtgaat gggcgccggc tgggggtggc agtacgcggt gaggctcact ccctccgaga
     2581 gtccaggagc gcccgagcgg agaggcggcc cgggagcagg ggggcggccc ccactccggc
     2641 cgggtgcccg gcccctggcc cctgcctgcc ctctagatcg ccgccgcagc cgggcgcgcc
     2701 gaagttggtc gtgaggcact gggcaggtaa gtatcaaggt tacaagacag gtttaaggag
     2761 accaatagaa actgggcttg tcgagacaga gaagactctt gcgtttctga taggcaccta
     2821 ttggtcttac tgacatccac tttgcctttc tctccacagg tgtccactcc caggcggccg
     2881 ccaccatgtc caacctgctg actgtgcacc aaaacctgcc tgccctccct gtggatgcca
     2941 cctctgatga agtcaggaag aacctgatgg acatgttcag ggacaggcag gccttctctg
     3001 aacacacctg gaagatgctc ctgtctgtgt gcagatcctg ggctgcctgg tgcaagctga
     3061 acaacaggaa atggttccct gctgaacctg aggatgtgag ggactacctc ctgtacctgc
     3121 aagccagagg cctggctgtg aagaccatcc aacagcacct gggccagctc aacatgctgc
     3181 acaggagatc tggcctgcct cgcccttctg actccaatgc tgtgtccctg gtgatgagga
     3241 gaatcagaaa ggagaatgtg gatgctgggg agagagccaa gcaggccctg gcctttgaac
     3301 gcactgactt tgaccaagtc agatccctga tggagaactc tgacagatgc caggacatca
     3361 ggaacctggc cttcctgggc attgcctaca acaccctgct gcgcattgcc gaaattgcca
     3421 gaatcagagt gaaggacatc tcccgcaccg atggtgggag aatgctgatc cacattggca
     3481 ggaccaagac cctggtgtcc acagctggtg tggagaaggc cctgtccctg ggggttacca
     3541 agctggtgga gagatggatc tctgtgtctg gtgtggctga tgaccccaac aactacctgt
     3601 tctgccgggt cagaaagaat ggtgtggctg ccccttctgc cacctcccaa ctgtccaccc
     3661 gggccctgga agggatcttt gaggccaccc accgcctgat ctatggtgcc aaggatgact
     3721 ctgggcagag atacctggcc tggtctggcc actctgccag agtgggtgct gccagggaca
     3781 tggccagggc tggtgtgtcc atccctgaaa tcatgcaggc tggtggctgg accaatgtga
     3841 acatagtgat gaactacatc agaaacctgg actctgagac tggggccatg gtgaggctgc
     3901 tcgaggatgg ggactgagcg gccgcgatcg cagacatgat aagatacatt gatgagtttg
     3961 gacaaaccac aactagaatg cagtgaaaaa aatgctttat ttgtgaaatt tgtgatgcta
     4021 ttgctttatt tgtaaccatt ataagctgca ataaacaagt taacaacaac aattgcattc
     4081 attttatgtt tcaggttcag ggggagatgt gggaggtttt ttaaagcaag taaaacctct
     4141 acaaatgtgg taggtcgaaa ttcccgataa ggatcttcct agagcatggc tacgtagata
     4201 agtagcatgg cgggttaatc attaactaca aggaacccct agtgatggag ttggccactc
     4261 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     4321 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ccttaattaa cctaattcac
     4381 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     4441 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     4501 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     4561 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     4621 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     4681 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     4741 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     4801 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     4861 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
     4921 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa
     4981 cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
     5041 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
     5101 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
     5161 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
     5221 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
     5281 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
     5341 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
     5401 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
     5461 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
     5521 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
     5581 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     5641 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     5701 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     5761 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     5821 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     5881 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     5941 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     6001 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     6061 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     6121 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     6181 ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga
     6241 gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt
     6301 tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata
     6361 cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac
     6421 cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg
     6481 ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg
     6541 tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag
     6601 cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct
     6661 ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc
     6721 aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt
     6781 ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg
     6841 tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga
     6901 gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     6961 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     7021 caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     7081 tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     7141 tgaccatgat tacgccagat ttaattaagg
//
