LOCUS       sequence-73219-e        6902 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with FEV (Ple67) promoter driving expression of iCre. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6902)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 6902)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS1990
FEATURES             Location/Qualifiers
     source          1..6902
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2459..2591
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2574..2593
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3672..3793
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3683..3702)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3737..3756
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3963..4092
                     /label=AAV2 ITR
     primer_bind     complement(4111..4128)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4111..4127)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4120..4142)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4269..4724
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4356..4375)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4566..4587
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4750..4854
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4855..4923,4924..5715)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5073..5092)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5886..6474
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6375..6394
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6762..6783
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6798..6803,6804..6821,6822..6828)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6836..6852
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6841..6863
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6860..6876
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6860..6876
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg ccggagaaga tcaagactca
      241 ggtgggtgga gatgactgcc ccccatgcac acatttcctg gtgctccacc ccctcagcaa
      301 atgacttctg ggtaagactg agggataaag cctatactgt tgatccttgc ccagctcaca
      361 gctgggatat ttgggtagac ctcactagga ccctctcctt tacaccttag cctcccactg
      421 agagcattaa gtgggcgaat tttgatgcct cccagctttc ccagctctta cctcctgccc
      481 ctggctattt tctgttctta cctcctgctc cttgttttct gtccatagtg ggtgggggaa
      541 gcagaaagag gaatgagaag gagagggatt gggatcttga ccccaacttc aaggccctca
      601 ccttctgtgg agccctgagt ttggtccaca gggaacattc caacctctag tcattgctcc
      661 caccagattc caggtccaca tcctgaaggc ttcatccctg tcccactagg atcctcccag
      721 gccctgggat ccctcttttt ccctccacac tctgcctccc actcttctcc ctggctcctt
      781 tgcaccctcc acgagcaaag atgaacactc cagaagttgc agcaaatgag ggtgaacatg
      841 ggccctgtag aaccccagaa gtaggagttt ccagccatcc tcaatcaggc atcccaggac
      901 caggcactta gcaggagacc tgcccattcc caggcctcct ccacccctgg gctcaagcag
      961 tgagagagga ctttgagaat gccagttttg acagtacccg ttccatactg caaggctgca
     1021 gaaggtgggc ttctgggctg gagcctcatc ttcagccagc atcctcctta gcctgcctct
     1081 ccagagccac gaagccccat aggtacccag agagactcca ggccacttgg gaaccacatc
     1141 aacatctttg catgagttag aaataggaat ccttttcttg gagtggattc cagccttgta
     1201 cagtgcacaa ccctgtctta acccctccac ctctccagcc tcgttcaggg atagccagaa
     1261 tgggagtagt ggtgagggtg ggtggcagac tgggaggaca ggggatgggg aaggatggga
     1321 gcagaatagt tgtacaggga agtagcaggg ggctcaggaa cccatcaggg aaagccagga
     1381 cagattctca acatctttag aggccttcag cagtccaagc cactactaat ttatggggac
     1441 tgaatgaatg agaaaaggag gggagggaga aggatgagag ggagagagaa ggctggaggg
     1501 gagatgagag tcggagagaa aagcctagga ggtaaagaga gacctagaag ggggcgggga
     1561 ggacagaaga gggggccacc catcataagt tgccaggaga gatggcccag caccaggcct
     1621 gaggcttggg ggccccagaa aggcaggaaa cgggccaaag caagaaggag acggggaagg
     1681 ggttcgggtc tctagggaag agcagagagt gggagaagag gaggagggag cggggatgcg
     1741 ggcagagata aagggagcaa cggctgcagg gccgggcgcg cagcgacagg cgagagggag
     1801 ggaagcggaa atttaaaagt gaagatgcag ataacgcagc ctggagacgg gaacccgggt
     1861 ggggatagag ggagggaggg ggaggagagt gagcggcgag tggagcggga gccgactgga
     1921 agaagggctc tagggagggg gctgtggctg ctggggtccg aggtggggcc gggtacacca
     1981 gccccatcac tgtttgcaga gagtcaggga ggcggaaaag acacgcgctc taggctccca
     2041 tcagggcaca tggcccggac ccatcccccg cgcgtctccc cggctgcggg gcgcgggggg
     2101 ctgccgggtg cgcttggctg tggcgcggcg cgttggagac tttattgcga tgggacgata
     2161 agaggggcgg gggcggggtc ctgggggccg aggcggcagc gctttaatta aaacggaaat
     2221 tgcggccccg ggccgcgcgg gggccggagg gttccaagcg gccccttagc tggaagcgtt
     2281 tctccaggac ccccccgcaa cccccgccac gcccgggctg ccccctcccg ccaggccctg
     2341 ccggacccgg cgccgtcttc tcctccttgt cacccgcggt cgcttcgggc ggggatcggt
     2401 gccaccgagc gcaaagcctg cctcggcgcg ccgaagttgg tcgtgaggca ctgggcaggt
     2461 aagtatcaag gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca
     2521 gagaagactc ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt
     2581 tctctccaca ggtgtccact cccaggcggc cgccaccatg tccaacctgc tgactgtgca
     2641 ccaaaacctg cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat
     2701 ggacatgttc agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt
     2761 gtgcagatcc tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc
     2821 tgaggatgtg agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat
     2881 ccaacagcac ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc
     2941 tgactccaat gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg
     3001 ggagagagcc aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct
     3061 gatggagaac tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta
     3121 caacaccctg ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac
     3181 cgatggtggg agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg
     3241 tgtggagaag gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc
     3301 tggtgtggct gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc
     3361 tgccccttct gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac
     3421 ccaccgcctg atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg
     3481 ccactctgcc agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga
     3541 aatcatgcag gctggtggct ggaccaatgt gaacatagtg atgaactaca tcagaaacct
     3601 ggactctgag actggggcca tggtgaggct gctcgaggat ggggactgag cggccgcgat
     3661 cgcagacatg ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa
     3721 aaaatgcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg
     3781 caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat
     3841 gtgggaggtt ttttaaagca agtaaaacct ctacaaatgt ggtaggtcga aattcccgat
     3901 aaggatcttc ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta
     3961 caaggaaccc ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga
     4021 ggccgggcga ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga
     4081 gcgagcgcgc agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg
     4141 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
     4201 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
     4261 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4321 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
     4381 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4441 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
     4501 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4561 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
     4621 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
     4681 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
     4741 ggaaatgtgc gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg
     4801 ctcatgagac aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt
     4861 attcaacatt tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt
     4921 gctcacccag aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg
     4981 ggttacatcg aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa
     5041 cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt
     5101 gacgccgggc aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag
     5161 tactcaccag tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt
     5221 gctgccataa ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga
     5281 ccgaaggagc taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt
     5341 tgggaaccgg agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta
     5401 gcaatggcaa caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg
     5461 caacaattaa tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc
     5521 cttccggctg gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt
     5581 atcattgcag cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg
     5641 gggagtcagg caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg
     5701 attaagcatt ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa
     5761 cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     5821 atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     5881 tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     5941 ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     6001 ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac
     6061 cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     6121 gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     6181 gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     6241 acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     6301 gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     6361 agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     6421 tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     6481 agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     6541 cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc
     6601 gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc
     6661 ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac
     6721 aggtttcccg actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact
     6781 cattaggcac cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg
     6841 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa
     6901 gg
//
