LOCUS       sequence-73222-e         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial S. thermophilus #1 Cas9 (ST1) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-ST1cas
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      318..345
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 gaagaagatc gcttggcctc gcgcgcagat cagttggaag aatttgtcca ctacgtgaaa
       61 ggcgagatca ccaaggtagt cggcaaataa tgtctaacaa ttcgttcaag ccgaggggcc
      121 gcaagatccg gccacgatga cccggtcgtc ggttcagggc agggtaccag gcacgcctaa
      181 ccgtcagtga gattggatga gtgaacgata ttgatcgaga agagccctgc gcagccgctg
      241 ccgtgcctgc aggaagcaac ggcccggagg gtggcgggca ggacgcccgc cataaactgc
      301 caggcatcaa attaagcaga aggccatcct gacggatggc ctttttgcgt ttctacaaac
      361 tctgctagct tctagagcac agctaacacc acgtcgtccc tatctgctgc cctaggtcta
      421 tgagtggttg ctggataact ttacgggcat gcataaggct cgtatgatat attcagggag
      481 accacaacgg tttccctcta caaataattt tgtttaactt ttactagagg aggaggcaaa
      541 aatgagcgac ctggtgctgg gcctggacat cggcatcggc agcgtgggcg tgggcatcct
      601 gaacaaggtg accggcgaga tcatccacaa gaacagtcgc atcttccctg ctgctcaggc
      661 tgagaacaac ctggtgcgcc gcaccaaccg ccagggtcgc cggcttgctc gccgcaagaa
      721 gcaccggcgc gtgcgcctga accgcctgtt cgaggagagc ggcctgatca ccgacttcac
      781 caagatcagc atcaacctga acccctacca gctgcgcgtg aa
//
