LOCUS       sequence-73223-e        7281 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with OLIG1 (Ple304) promoter driving expression of iCre.
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7281)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7281)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1993
FEATURES             Location/Qualifiers
     source          1..7281
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2838..2970
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2953..2972
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4051..4172
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4062..4081)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4116..4135
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4342..4471
                     /label=AAV2 ITR
     primer_bind     complement(4490..4507)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4490..4506)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4499..4521)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4648..5103
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4735..4754)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4945..4966
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5129..5233
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5234..5302,5303..6094)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5452..5471)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6265..6853
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6754..6773
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7141..7162
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7177..7182,7183..7200,7201..7207)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7215..7231
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7220..7242
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7239..7255
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7239..7255
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac tgagttaata tttccaggtc agagcgcctc
      241 cgagagaaaa aggcggtggc cttggagctc tggtctcctt aactgcaccc tgggaagtct
      301 tcacacggcc tttcttcccc tggtggtcta atctggagtc agatccagcc gtcagctctt
      361 acagacgggg aaggaccggg agccaacggg aaaacaacag gtgagcttgt gaacaagtcc
      421 ccttctgtgt ctcgggcagg gtgtctgccc gggcaaccgt ggggctgcac tgacttccag
      481 aaccttctgc ctctgcaggt gttttaggaa cttggcctca gcgcttcagt gacccatatt
      541 ttattacctt tcaggtttct ataattcatc attcactcct ttctgaaaac ctagaatgaa
      601 aaagtttagc agctcagcgc cgcggcctct ctccattctc tccccaaaca catcggcctg
      661 tttgttctct ttcatccccg ctcttcctgt tgcattctgc cggttcaatg gggaaggagt
      721 gaaaccggat ggctggaccc agtgcgggga aatgacttca gagccacctt tgtttccctc
      781 attcctttga gcgcacttgg ccctgcgtct ctatgaaagg gcttgtttga accattcgga
      841 acatccagca agccagagat gctgcggcca gaaccgctcg catcttttca ggctgcctga
      901 gactctgggt taaactgcca aatttttgca acaaaactaa ccatgacatt ggacattgtg
      961 ttataaatta gccacagcct tccaagcaaa ttgtctcttt ttattgtatc agtgtaagcc
     1021 tcggaaacat ttggcttcag ctactacctt cagggaggct cagaggatat ttttgttctg
     1081 ccccaaacca actcttcaga caccaccttc cttgtcctca cgcttgacta gcagacaatg
     1141 ggttcttcag aagccacagc atttcagctg ttttggcccc agaggccaca agctgactgc
     1201 atgtcattct ccaccagcag agcgtcacct cggggtagct ccaaacagta tcaaccggtt
     1261 tgtggtgagt ggataaacac caggctgggt gaatgaagtc acaggctgag tcatcctctg
     1321 cacatggggg ctgaatgggg ctcagtcagg cccagaggag cctccccggc aaggtgctgg
     1381 gggccaggct ctccctgcca gtgaggctgg ggtccgtccc caatagtcat tcctttggcc
     1441 aacagaaatg ataaggcaga caaatggaag atcaatgaag caacatcgtg gcaataatgc
     1501 aattggtcct gccgtagtca tgaacatgta aataacaaga cagcaataag aagaccccag
     1561 actctgccta gaaacaaaat attcagcctg ttgttgttgt ttcttattgc aggattttga
     1621 gtggaaaagt agttccactt gtcaatcagc tacgttgcag gggctaatag attctggtgt
     1681 ttagggaggt caacacggaa tttaatttaa catctcacaa tacacccaca tgcccagaaa
     1741 atgtggacag cttgataggg ttcactgagc caaagaagca agacccaaac agtgcattaa
     1801 tgtatagggg aagaatagtt gcaatcagcc ccttgtgaat tggacagagg gcttggagat
     1861 gaagcaatta tcagccatcg gggattaata gtaaagtgtg aataccaagc tgtatatgtt
     1921 aggggcagaa tatgttgcct atagaaaaca acaaaggacg tttcttacta ttaagtgaga
     1981 ttaaacgtaa tggcctgatt taatcacaaa ggcagaatta aattcagatg gcaacttcaa
     2041 agtggacaca ggaacacagg ccactgctta catttacata tagctggcat agcttggcct
     2101 gcaacacaat aggataaagt ttctttggcc acttaaacaa ttttctattc ttcacctttt
     2161 tctttttttc tttttttttt cttttgtggg tgtgtgtgtg tgtgtgtgtg tgtgtgaaca
     2221 ctcactgtat ttattcccca attgcattta gttagtaaga aggagctaag tgttatattt
     2281 attcatggag gctataactg ttaattcttc caaattgtga aaaaaaagcc tcatagggtc
     2341 aataagaaca gagtagttat aatcagattg tcaccaaaaa agaaaaagaa gaaaaaggtt
     2401 gtttttgagt agacagttgc tggaggaagg cacgtgagtt gcagcctgac cagtgcgacc
     2461 agtcctcctt caacagcccc gcttgccttg aaaaggcgcc cttactccgc tcagggggcc
     2521 tgtcccgcgc ggctggcaca ggcgctcaca ataacctcct aaagcggtgc cagccgcacc
     2581 tccgcgcggc cccggcacaa gcagccaatg aacacgcggc tgcgcccggc ctcgcgcctc
     2641 cattggctgc gccccgccac ccgctgcccc gcaggttccc aagccgggtt taaaggggta
     2701 gggcgcgggc cagggcccca ccatcgtttc cccgcgcgca ggtccgcggg gaggggcggc
     2761 ctgccgaccg gcccacccca gggcgttcct gaagggcgtc ctcggcgcgc cgaagttggt
     2821 cgtgaggcac tgggcaggta agtatcaagg ttacaagaca ggtttaagga gaccaataga
     2881 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
     2941 ctgacatcca ctttgccttt ctctccacag gtgtccactc ccaggcggcc gccaccatgt
     3001 ccaacctgct gactgtgcac caaaacctgc ctgccctccc tgtggatgcc acctctgatg
     3061 aagtcaggaa gaacctgatg gacatgttca gggacaggca ggccttctct gaacacacct
     3121 ggaagatgct cctgtctgtg tgcagatcct gggctgcctg gtgcaagctg aacaacagga
     3181 aatggttccc tgctgaacct gaggatgtga gggactacct cctgtacctg caagccagag
     3241 gcctggctgt gaagaccatc caacagcacc tgggccagct caacatgctg cacaggagat
     3301 ctggcctgcc tcgcccttct gactccaatg ctgtgtccct ggtgatgagg agaatcagaa
     3361 aggagaatgt ggatgctggg gagagagcca agcaggccct ggcctttgaa cgcactgact
     3421 ttgaccaagt cagatccctg atggagaact ctgacagatg ccaggacatc aggaacctgg
     3481 ccttcctggg cattgcctac aacaccctgc tgcgcattgc cgaaattgcc agaatcagag
     3541 tgaaggacat ctcccgcacc gatggtggga gaatgctgat ccacattggc aggaccaaga
     3601 ccctggtgtc cacagctggt gtggagaagg ccctgtccct gggggttacc aagctggtgg
     3661 agagatggat ctctgtgtct ggtgtggctg atgaccccaa caactacctg ttctgccggg
     3721 tcagaaagaa tggtgtggct gccccttctg ccacctccca actgtccacc cgggccctgg
     3781 aagggatctt tgaggccacc caccgcctga tctatggtgc caaggatgac tctgggcaga
     3841 gatacctggc ctggtctggc cactctgcca gagtgggtgc tgccagggac atggccaggg
     3901 ctggtgtgtc catccctgaa atcatgcagg ctggtggctg gaccaatgtg aacatagtga
     3961 tgaactacat cagaaacctg gactctgaga ctggggccat ggtgaggctg ctcgaggatg
     4021 gggactgagc ggccgcgatc gcagacatga taagatacat tgatgagttt ggacaaacca
     4081 caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct attgctttat
     4141 ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt cattttatgt
     4201 ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc tacaaatgtg
     4261 gtaggtcgaa attcccgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg
     4321 gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc
     4381 gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg ggctttgccc
     4441 gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca ctggccgtcg
     4501 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     4561 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     4621 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     4681 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     4741 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     4801 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     4861 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     4921 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     4981 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     5041 aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta acgcttacaa
     5101 tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat
     5161 acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg
     5221 aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc
     5281 attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga
     5341 tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga
     5401 gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg
     5461 cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca tacactattc
     5521 tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac
     5581 agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact
     5641 tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca
     5701 tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg
     5761 tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa ctggcgaact
     5821 acttactcta gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg
     5881 accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg
     5941 tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat
     6001 cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc
     6061 tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat
     6121 actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt
     6181 tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc
     6241 cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt
     6301 gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac
     6361 tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg ttcttctagt
     6421 gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct
     6481 gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga
     6541 ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac
     6601 acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg
     6661 agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt
     6721 cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc
     6781 tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg
     6841 gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc
     6901 ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc
     6961 ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag
     7021 cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt ggccgattca
     7081 ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat
     7141 taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc ttccggctcg
     7201 tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct atgaccatga
     7261 ttacgccaga tttaattaag g
//
