LOCUS       sequence-73224-e        7672 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with S100B (Ple266) promoter driving expression of 
            iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7672)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7672)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS1994
FEATURES             Location/Qualifiers
     source          1..7672
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          3229..3361
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3344..3363
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    4442..4563
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4453..4472)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4507..4526
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4733..4862
                     /label=AAV2 ITR
     primer_bind     complement(4881..4898)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4881..4897)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4890..4912)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5039..5494
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5126..5145)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5336..5357
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5520..5624
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5625..5693,5694..6485)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5843..5862)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6656..7244
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7145..7164
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7532..7553
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7568..7573,7574..7591,7592..7598)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7606..7622
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7611..7633
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7630..7646
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7630..7646
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtcagatg tgagttaagc agtttgaaca
      241 atcgattgat tagacttttg tggctgctca tttgggcatg tggccgagta ctgtgctgtc
      301 ctggcccaca gcgtcctaca ggaagggacg ggctgtaaga cgtgggatct tctgaagtcg
      361 tcaaatgaaa aacactcctg ttgagtgaaa aaacaaatag gctaatccta tgatttttta
      421 aacatttaaa cctgacatga ttttattgca aatttccaag aatccttaaa agttataaaa
      481 agcataatca aaataaatat ctgtgacaga gttctacaaa agggaaaact cagttttcct
      541 tttgagatct tgccccagca tcagttcaaa cacttcctca tatatttcac caagactcag
      601 ttcctgagca agaaaccaca ggcacagcaa gtgccatgaa aagcggcttt gtgtggggtg
      661 ggctcttcac actccaatct ccacttcctt ctcaaggcct caaaaaaagt tgaaaaatga
      721 aaacaaaagc cctgctgtgt tgagctgggc tctggcgttg ccatggaccc agggcaaaca
      781 gcggtgctcc tgctctgccc ccggctcagc tcatgctggg cctgcacttc tggaagggag
      841 catggacttt ggaatgactg gttagaaccc aaatgaatta atggaatttg acatagttca
      901 aaaataataa aatgtgatac ccatgaaatg ctgatattct gccttaattt gccagattgg
      961 gggcctcctg gatttagagt attctaaagt agccagtagg ttcactctgc ccttttagac
     1021 ctaatatatc tggctcagaa agaagtctct gtccctctgc tatttgctgt tctttgcttt
     1081 gcccagtctg actcctgcca gctccaggtt ctgcagacaa gaacagtaac ttttataata
     1141 ataataatga ctcccaagtg agtgcattgg gcccttcacg tgtgtctgta tgtgtgcaca
     1201 tgtatgtgcg tgtcaatttc ctttactcct cctacagccc tctcaggggt gcagcttttt
     1261 ttctgtcttt tactcagcct gagaaaggtt gtcgtttgac aaggtttgtt cagaggtcgg
     1321 atctgaatcc agctccaagg ccccagcacc caagccctga ccccatggct gcctgctggc
     1381 tgggagtggc attctttaga acaggaaagg cagtgagttc tcattgcatc aatacttgca
     1441 tttgctacaa cagaagcttt ttgtctcttc ctcacattcc tttagaacac aagcctcctt
     1501 ttctgcctgt ggccatcggt cactggagtc agcctcgtga gtggcttggt ggcggatggc
     1561 accgtccttt gtgggagaaa acaatgttgc tgcccaggcc tttctggaat gaccccttcc
     1621 caccccctga ccagccccag caaggcccgg ggctggctgc ctagttgatt ggagccaaga
     1681 gtttgctgaa tggatgaagg gagaagggac atccaagtga ctgaagaaga tcttaacaga
     1741 agattgatat attcaaatag aggcccctgt tgacccacaa gctccttcgt gctctggcct
     1801 gggcaggcag cgtggtcagc acttacagga atggtccctc caacatctga gcgaggaagg
     1861 ggcacagagc acagttgtgc cttccttggg cctgggcaag ctctgtgctt cacagagcaa
     1921 gcctgtgtca gatccttcag cagagaccac agccaggggt ggtgaagccg atgaggaagt
     1981 gagggccaac cacactgctc aggctgttcc tcacaggagg ctggggcagg ctgagccacg
     2041 caagaccaca gctctcagcc ctgtgctatt tccagggcac acttacagat cctgaatggt
     2101 agagaacgtc ctggataaaa caatgacagc aagacagcca tgtacacaga tatcttgtgg
     2161 gaaacccttt cacggccact ttgaaactgg gttgggagaa ggacgtgatg ctgggtcagg
     2221 catccattgg ggaccatgga catttttgga gagtttgggc caatgttctt gtgtaatttg
     2281 tcagaaggaa cacctacttc ttcattcttg tgaagaagaa cccataaact ggcccccgtg
     2341 ttcagaggct ccagtcgagc gagttgaggg gacagctgga cttctccttc ctcaggctgg
     2401 ctggaggctt cagcaagggg gcacaccgtc cacacgcctc tgtaaaacag cgttcgaacg
     2461 ggacatctct aaggcatcgt ccaactctga gagtctgtga ccttccaccc tggggtggag
     2521 ggaaaggcct tgggatacag tccacacatc acgttttccc ctaaaagtac cacccgtttt
     2581 agtccctgca gacactggcc tcagcagctt ctctgaggca gccccgttga gcccagggct
     2641 gaccacagac aagcactact gtccacagaa cttcacgccc agtggggcca gacttggagg
     2701 atggcagagg agagaagctc caggggcctc tccattagaa accaacttgc aggccactta
     2761 atctcctatg ctcagcctgt acttggaagc tgcttgttct tggctgcaca tttgctttgt
     2821 ttgaatcaat taatccaagt ctctctcctc tccccacccc caaccttgcc tttagggtga
     2881 catcaatatt catgtaataa agaaatcgca caaaaagctg actccccact tcctgcccta
     2941 caggcccttt tttcttcctc agcccatgtg caatcctggc tcccagcaag tccccggggc
     3001 tgcttggtca atgcagccct gtgtgcaggc ctggcagccc tgccaccccc gccttcggct
     3061 cccattggct gccacggcct gcagtgggct gcaccagggt tcatccatcc tccctgggca
     3121 gagggaataa gaggctgcct ctgcccacca gtcctgccgc ccaggacccg cagcagagac
     3181 gacgcctgca gcaaggcgcg ccgaagttgg tcgtgaggca ctgggcaggt aagtatcaag
     3241 gttacaagac aggtttaagg agaccaatag aaactgggct tgtcgagaca gagaagactc
     3301 ttgcgtttct gataggcacc tattggtctt actgacatcc actttgcctt tctctccaca
     3361 ggtgtccact cccaggcggc cgccaccatg tccaacctgc tgactgtgca ccaaaacctg
     3421 cctgccctcc ctgtggatgc cacctctgat gaagtcagga agaacctgat ggacatgttc
     3481 agggacaggc aggccttctc tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc
     3541 tgggctgcct ggtgcaagct gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg
     3601 agggactacc tcctgtacct gcaagccaga ggcctggctg tgaagaccat ccaacagcac
     3661 ctgggccagc tcaacatgct gcacaggaga tctggcctgc ctcgcccttc tgactccaat
     3721 gctgtgtccc tggtgatgag gagaatcaga aaggagaatg tggatgctgg ggagagagcc
     3781 aagcaggccc tggcctttga acgcactgac tttgaccaag tcagatccct gatggagaac
     3841 tctgacagat gccaggacat caggaacctg gccttcctgg gcattgccta caacaccctg
     3901 ctgcgcattg ccgaaattgc cagaatcaga gtgaaggaca tctcccgcac cgatggtggg
     3961 agaatgctga tccacattgg caggaccaag accctggtgt ccacagctgg tgtggagaag
     4021 gccctgtccc tgggggttac caagctggtg gagagatgga tctctgtgtc tggtgtggct
     4081 gatgacccca acaactacct gttctgccgg gtcagaaaga atggtgtggc tgccccttct
     4141 gccacctccc aactgtccac ccgggccctg gaagggatct ttgaggccac ccaccgcctg
     4201 atctatggtg ccaaggatga ctctgggcag agatacctgg cctggtctgg ccactctgcc
     4261 agagtgggtg ctgccaggga catggccagg gctggtgtgt ccatccctga aatcatgcag
     4321 gctggtggct ggaccaatgt gaacatagtg atgaactaca tcagaaacct ggactctgag
     4381 actggggcca tggtgaggct gctcgaggat ggggactgag cggccgcgat cgcagacatg
     4441 ataagataca ttgatgagtt tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt
     4501 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca ttataagctg caataaacaa
     4561 gttaacaaca acaattgcat tcattttatg tttcaggttc agggggagat gtgggaggtt
     4621 ttttaaagca agtaaaacct ctacaaatgt ggtaggtcga aattcccgat aaggatcttc
     4681 ctagagcatg gctacgtaga taagtagcat ggcgggttaa tcattaacta caaggaaccc
     4741 ctagtgatgg agttggccac tccctctctg cgcgctcgct cgctcactga ggccgggcga
     4801 ccaaaggtcg cccgacgccc gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc
     4861 agccttaatt aacctaattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     4921 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     4981 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     5041 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     5101 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     5161 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     5221 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     5281 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     5341 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     5401 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     5461 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     5521 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5581 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5641 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5701 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5761 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5821 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5881 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5941 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     6001 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     6061 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     6121 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6181 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6241 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6301 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6361 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6421 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6481 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6541 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6601 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6661 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6721 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6781 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6841 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6901 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6961 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     7021 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     7081 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     7141 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7201 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7261 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     7321 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     7381 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     7441 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     7501 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     7561 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     7621 aatttcacac aggaaacagc tatgaccatg attacgccag atttaattaa gg
//
