LOCUS       sequence-73229-e        7044 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with DCX (Ple302) promoter driving expression of iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7044)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7044)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2021
FEATURES             Location/Qualifiers
     source          1..7044
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          2602..2734
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2717..2736
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3815..3936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3826..3845)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3880..3899
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4105..4234
                     /label=AAV2 ITR
     primer_bind     complement(4253..4270)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4253..4269)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4262..4284)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4411..4866
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4498..4517)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4708..4729
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4892..4996
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4997..5065,5066..5857)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5215..5234)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6028..6616
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6517..6536
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6904..6925
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6940..6945,6946..6963,6964..6970)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6978..6994
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6983..7005
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7002..7018
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7002..7018
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccga tgtccttttt acttctatct tccataatat
      241 tctaaaatta ttttcccttg ttttgttcct atcctacttc ctcttagtct actttgttga
      301 cttcattaaa aaacaaaaaa ccagttgttg gatacttgag ctaaactgcc ttaaagaatc
      361 tgcagatttt attttatttt ttttctctca agagggtaaa aggaagagag ctacaatttc
      421 taagaagcct ggcttggctg tctgagtctg gcccccaggc agattaggcc aaggttttgg
      481 ccaagtgaaa ttgccaattt tctaaaagaa agggctagca cattgctcat tagagcattc
      541 tgattttgtc tgcgcaatct ttctgctacc ccgcaatttc ctgttggtta taaatgaaac
      601 ctttctagct gttaatgcag cctgtgaatt tttttaaaag catgtaatta atcataggag
      661 gttgggggga ttcactaagc ctgagttaca tgggagaagc tggacaaggc actaggacct
      721 agaaggcatc tatccaccct ggcaggaatt tcttgcttgg agctcagaca acaaaggcat
      781 agagagattg gttttctttc tctcagcatc tccacccaac cagcagaaaa ccggtgagtg
      841 gggcttttaa gtgattttca agaagaatgt aacagatgtc aaacgggaaa agcacaaggc
      901 aaagcctgct ctctctgtct ctctgtctcc tcttctcctt ttttgcctta ttctatccga
      961 ttttttccct aagcttctac ctgggatttt cctttggaaa agtgagtttg atgttccttt
     1021 gttttcactg tgatgttaat ttagaataat actacctctg atcctaaagc aaagcaaagc
     1081 cttactggca tgcctgggga aatgtttgct gcttgccttg aggaggtggg gtctcttacc
     1141 actgcaggtt gtctgacaga gacaatgctg agctcagcat aggtcatggt gacattggaa
     1201 aaaaggcgga attgagcctg gcagacccat taggcaccag tctttcttat ctcctgtcct
     1261 cctggtccct tgcaaatata ttgatgtggc agtgtgtagc agctgagccc tgcttgcttt
     1321 gtgagtcctt ttatccccat ctgtgagatg catgttaata gtttggctcg taggatgtca
     1381 ctacatttgc tagcatttgt ggcttcagtt gtattgggtt tcatgttttg attgtttggg
     1441 gttcttggtg ggggaggggg ttcaacagaa gggagaaaag caaagcctga caaatgacca
     1501 tcttttctca gctaatgcac ctgggcaata tacaagtttg gggtgaattg cctgctgtga
     1561 gggtaaatgt cacttcaatt aaggtagaaa cccagaacaa tgaaaggtgt gcttccttct
     1621 aaaggtcccg tatgctgttc ggagagtcat ttgtgaatct ttcaacaatt aaattattcc
     1681 attaagaggt gttgctgcat cagtggggag ggggtggagc acctgggggg gaaaaaaaag
     1741 gattttgtga acaaatggaa ccgggggaag acagagctag taacttgtta aataacttat
     1801 ttttctaatc ctttttcccc ccagcttatt tcttatgaat gtcggatagc tgcaccagct
     1861 tggtggggaa agggtttgat gaatagcaca aagacactgg ctgttccctg gaggctgtcc
     1921 ctttaaagga gaatcttagt ttattctggg gggaggggat gcacacatta gagtaggaaa
     1981 gagggcttgg aataaaatga aaacactccc ccttcatagt cattgtactg aaatgcaaag
     2041 actgcttcct aagctggaga tgctaacctt gggtagctcc ttctgttctc ttcaagggga
     2101 attttgtcag gctatggatt catttacaac tgttagtcat gtgggcatgt gtgaggaaac
     2161 agatgccagt tttaatgtat ttagcccgaa gttccaattt gataggagcc actgtcagta
     2221 agtctcagga ttttcagcta tttcaaaatc tccccttctc ctctgtctgg aacagtgcca
     2281 agagtgcctc cctctctatc tcttactccc aacccccaca accaccagca cccccgccca
     2341 gcccctcctt cttctctatt aagatcaata ttcctgcagg tcaggggcaa gcagcagatg
     2401 ggtcacaggc ttttttcaac cagttctttt cacaagcagc agattgcaga tctggatctg
     2461 gctaatattt aaaatccctt cttttttcct tctccttgtc cctttttgtt tttgcctctc
     2521 ttcaccccca tccctttctc ccacgctcag gtctctgagg ttccaccggc gcgccgaagt
     2581 tggtcgtgag gcactgggca ggtaagtatc aaggttacaa gacaggttta aggagaccaa
     2641 tagaaactgg gcttgtcgag acagagaaga ctcttgcgtt tctgataggc acctattggt
     2701 cttactgaca tccactttgc ctttctctcc acaggtgtcc actcccaggc ggccgccacc
     2761 atgtccaacc tgctgactgt gcaccaaaac ctgcctgccc tccctgtgga tgccacctct
     2821 gatgaagtca ggaagaacct gatggacatg ttcagggaca ggcaggcctt ctctgaacac
     2881 acctggaaga tgctcctgtc tgtgtgcaga tcctgggctg cctggtgcaa gctgaacaac
     2941 aggaaatggt tccctgctga acctgaggat gtgagggact acctcctgta cctgcaagcc
     3001 agaggcctgg ctgtgaagac catccaacag cacctgggcc agctcaacat gctgcacagg
     3061 agatctggcc tgcctcgccc ttctgactcc aatgctgtgt ccctggtgat gaggagaatc
     3121 agaaaggaga atgtggatgc tggggagaga gccaagcagg ccctggcctt tgaacgcact
     3181 gactttgacc aagtcagatc cctgatggag aactctgaca gatgccagga catcaggaac
     3241 ctggccttcc tgggcattgc ctacaacacc ctgctgcgca ttgccgaaat tgccagaatc
     3301 agagtgaagg acatctcccg caccgatggt gggagaatgc tgatccacat tggcaggacc
     3361 aagaccctgg tgtccacagc tggtgtggag aaggccctgt ccctgggggt taccaagctg
     3421 gtggagagat ggatctctgt gtctggtgtg gctgatgacc ccaacaacta cctgttctgc
     3481 cgggtcagaa agaatggtgt ggctgcccct tctgccacct cccaactgtc cacccgggcc
     3541 ctggaaggga tctttgaggc cacccaccgc ctgatctatg gtgccaagga tgactctggg
     3601 cagagatacc tggcctggtc tggccactct gccagagtgg gtgctgccag ggacatggcc
     3661 agggctggtg tgtccatccc tgaaatcatg caggctggtg gctggaccaa tgtgaacata
     3721 gtgatgaact acatcagaaa cctggactct gagactgggg ccatggtgag gctgctcgag
     3781 gatggggact gagcggccgc gatcgcagac atgataagat acattgatga gtttggacaa
     3841 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     3901 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     3961 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     4021 tgtggtagtc gaaattcccg ataaggatct tcctagagca tggctacgta gataagtagc
     4081 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     4141 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4201 cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg
     4261 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4321 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4381 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4441 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4501 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4561 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4621 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4681 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4741 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     4801 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     4861 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     4921 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     4981 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5041 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5101 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5161 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5221 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5281 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5341 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5401 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5461 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5521 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5581 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     5641 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     5701 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     5761 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     5821 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     5881 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     5941 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6001 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6061 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6121 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6181 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6241 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6301 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6361 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6421 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6481 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6541 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6601 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     6661 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     6721 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     6781 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     6841 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     6901 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     6961 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7021 tgattacgcc agatttaatt aagg
//
