LOCUS       sequence-73231-e        7035 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with hs1218 promoter driving expression of iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7035)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2023
FEATURES             Location/Qualifiers
     source          1..7035
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     regulatory      2552..2561
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          2593..2725
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2708..2727
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3806..3927
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3817..3836)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3871..3890
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4096..4225
                     /label=AAV2 ITR
     primer_bind     complement(4244..4261)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4244..4260)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4253..4275)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4402..4857
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4489..4508)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4699..4720
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4883..4987
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4988..5056,5057..5848)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5206..5225)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6019..6607
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6508..6527
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6895..6916
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6931..6936,6937..6954,6955..6961)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6969..6985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6974..6996
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6993..7009
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6993..7009
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg tgctgctgta tagaatgttc
      241 aagattctac gtgctttgtg aatcagtttt cagtatgctt caaatccatc aagggaaaat
      301 gagggccatg attcttccaa atggaatcat taactaagtt tgaaagctca gagaattaaa
      361 ccaagtttgt catgacctca gtttgaaaaa ttcagagaat taaactgagt ctcttgtgag
      421 acagagaaca agtagaaact aggcttcaga aaggaagtaa tagtagaggc cactgaagta
      481 agtcaccatt gacctatgtc cctctcaatg aagtttcttc tttacagtta gttcctgcag
      541 aaaaatatat tcagtgcaat atattagtta tgggtccaac agcacagttc aaaacatgtg
      601 tagctgatgt atgttttccc ccaaaaatac aagtattggg ccgttcattg tcatctacac
      661 aaagaactga gtcctaaaat cctttactca actaaattct taataagtaa actgagacta
      721 accctctaat accagatttg ttaaacaaca tgatttaggt ccttgctatt catgcacctt
      781 gtacaactaa aatgactggt aaagctagaa tcatgaaaga gtcattttat gtagctgcta
      841 aattcctctc aaatctcctt gcaacttaac gagtaaagaa agctcaatgt gtaatgccgg
      901 ctaatgtggt ttatgctaga aaatagtatc aacctatctt tgtagcttat ctaagccagg
      961 ataatacttt tagcctttat agtagaccca actccatttt ttcaactctt gactatgtat
     1021 ttcctgaaat ccttttccaa agagttaaat tggatgtgaa atctgtacta ggctatggag
     1081 ctgtctttgt tctaagtctc gccagcaaag cctcgcctct gtatcacttt cattgcgttc
     1141 tgattgagct tgacagtgtg acaggaaagc aaagggaggc aattccaaac tgaatgagag
     1201 tctttttcag ccacacagca gccttctcct tagcccctaa gaaaaagagg taacagatgc
     1261 cgatagcaaa gcatccctaa caaagtctta actttgatgc aggcagaaga agaaaaaaat
     1321 gtaaaagatt gtctcagcgc aggaaaacct tgaatggaac acaaaggaag caaaatgaga
     1381 aaaatcgtgg ttcagaaaat ggctgggaga gttatgtagc tatcgtgcgt ttaagaaatt
     1441 attttagtct caacaagagg ttaggatctg cagaatctgc tctgccaggt atggcacaac
     1501 tggaatagtt gcaatatatg gggcccgagc aaaaggtgat cagtgatgct gtgattgctt
     1561 ggcatggagg atgttcatga tgaatgccct gtcccttggg tggaaagtaa aactccattg
     1621 aaatgacaac tttatagtat agccattacc tttgtactct ggggagtaga tcttttctga
     1681 gagctccagg aacatccaaa ctgagcagcc ggggtccccc ccacccccca ccccgcccca
     1741 cgcggcaact ttgagcctgt gctgggacag agcctctagt tcctaaatta gtccatgagg
     1801 tcagaggcag cactgccatt gtaacgcgat tggagaggat cacgtcaccg gacacgcccc
     1861 caggcatctc cctgggtctc ctaaacttgg cggggagaag ttttagccct taagttttag
     1921 cctttaaccc ccatattcag aactgtgcga gttggcgaaa ccccacaaat cacaacaaac
     1981 tgtacacaac accgagctag aggtgatctt tcttgtccat tccacacagg ccttagtaat
     2041 gcgtcgccat agcaacagtg tcactagtag caccagcact tccccacacc ctccccctca
     2101 ggaatccgta ctctccagtg aaccccagaa acctctggag agttctggac aagggcggaa
     2161 cccacaactc cgattactca agggaggcgg ggaagctcca ccagacgcga aactgctgga
     2221 agattcctgg ccccaaggcc tcctccggct cgctgattgg cccagcggag agtgggcggg
     2281 gccggtgaag actccttaaa ggcgcagggc ggcgagcagg tcaccagacg ctgacagcta
     2341 ctcagaacca aatctggttc catccagaga caagcgaaga caagagaagc agagcgagcg
     2401 gcgcgttccc gatcctcggc caggaccagc cttccccaga gcatccctgc cgcggagcgc
     2461 aaccttccca ggagcatccc tgccgcggag cgcaactttc cccggagcat ccacgccgcg
     2521 gagcgcagcc ttccagaagc agagcgcggc gccatggcgg cgcgccgaag ttggtcgtga
     2581 ggcactgggc aggtaagtat caaggttaca agacaggttt aaggagacca atagaaactg
     2641 ggcttgtcga gacagagaag actcttgcgt ttctgatagg cacctattgg tcttactgac
     2701 atccactttg cctttctctc cacaggtgtc cactcccagg cggccgccac catgtccaac
     2761 ctgctgactg tgcaccaaaa cctgcctgcc ctccctgtgg atgccacctc tgatgaagtc
     2821 aggaagaacc tgatggacat gttcagggac aggcaggcct tctctgaaca cacctggaag
     2881 atgctcctgt ctgtgtgcag atcctgggct gcctggtgca agctgaacaa caggaaatgg
     2941 ttccctgctg aacctgagga tgtgagggac tacctcctgt acctgcaagc cagaggcctg
     3001 gctgtgaaga ccatccaaca gcacctgggc cagctcaaca tgctgcacag gagatctggc
     3061 ctgcctcgcc cttctgactc caatgctgtg tccctggtga tgaggagaat cagaaaggag
     3121 aatgtggatg ctggggagag agccaagcag gccctggcct ttgaacgcac tgactttgac
     3181 caagtcagat ccctgatgga gaactctgac agatgccagg acatcaggaa cctggccttc
     3241 ctgggcattg cctacaacac cctgctgcgc attgccgaaa ttgccagaat cagagtgaag
     3301 gacatctccc gcaccgatgg tgggagaatg ctgatccaca ttggcaggac caagaccctg
     3361 gtgtccacag ctggtgtgga gaaggccctg tccctggggg ttaccaagct ggtggagaga
     3421 tggatctctg tgtctggtgt ggctgatgac cccaacaact acctgttctg ccgggtcaga
     3481 aagaatggtg tggctgcccc ttctgccacc tcccaactgt ccacccgggc cctggaaggg
     3541 atctttgagg ccacccaccg cctgatctat ggtgccaagg atgactctgg gcagagatac
     3601 ctggcctggt ctggccactc tgccagagtg ggtgctgcca gggacatggc cagggctggt
     3661 gtgtccatcc ctgaaatcat gcaggctggt ggctggacca atgtgaacat agtgatgaac
     3721 tacatcagaa acctggactc tgagactggg gccatggtga ggctgctcga ggatggggac
     3781 tgagcggccg cgatcgcaga catgataaga tacattgatg agtttggaca aaccacaact
     3841 agaatgcagt gaaaaaaatg ctttatttgt gaaatttgtg atgctattgc tttatttgta
     3901 accattataa gctgcaataa acaagttaac aacaacaatt gcattcattt tatgtttcag
     3961 gttcaggggg agatgtggga ggttttttaa agcaagtaaa acctctacaa atgtggtagt
     4021 cgaaattccc gataaggatc ttcctagagc atggctacgt agataagtag catggcgggt
     4081 taatcattaa ctacaaggaa cccctagtga tggagttggc cactccctct ctgcgcgctc
     4141 gctcgctcac tgaggccggg cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg
     4201 cctcagtgag cgagcgagcg cgcagcctta attaacctaa ttcactggcc gtcgttttac
     4261 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     4321 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     4381 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     4441 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     4501 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     4561 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     4621 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     4681 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     4741 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     4801 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     4861 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4921 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4981 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     5041 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5101 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5161 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5221 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5281 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5341 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5401 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5461 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5521 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5581 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5641 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5701 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5761 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5821 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5881 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5941 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     6001 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6061 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6121 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6181 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6241 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6301 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6361 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6421 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6481 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6541 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6601 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6661 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     6721 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     6781 agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg
     6841 cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt
     6901 gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt
     6961 gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc atgattacgc
     7021 cagatttaat taagg
//
