LOCUS       sequence-73232-e        6827 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with hs671 promoter driving expression of iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6827)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 6827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2024
FEATURES             Location/Qualifiers
     source          1..6827
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     CDS             complement(282..299)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     regulatory      2344..2353
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     intron          2385..2517
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2500..2519
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3598..3719
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3609..3628)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3663..3682
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   3888..4017
                     /label=AAV2 ITR
     primer_bind     complement(4036..4053)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4036..4052)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4045..4067)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4194..4649
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4281..4300)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4491..4512
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4675..4779
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4780..4848,4849..5640)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4998..5017)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5811..6399
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6300..6319
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6687..6708
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6723..6728,6729..6746,6747..6753)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6761..6777
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6766..6788
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6785..6801
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6785..6801
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtggcgcg tgctgagatt gtgtgatgtg
      241 gagaaaagga agatatgcag agatgttagc atggccacag ggtgatggtg gtggtggtgc
      301 tggggcaagt gtgcactact aataggagaa gatgtcaaaa aaaatgaagc ataataatag
      361 tgcaatttat ccttgctaaa cgtactgcag tgtgctataa ttaaatatga aaacctaaat
      421 ttatctctat gttcggttgt cattaattta acagggagtg cacatgttct aatctaatgg
      481 ttgatttttt ttctccatgt gcagcatatt aaaatgtata ttagcctcaa ggtgcataaa
      541 cacaaaataa tagaccaaag gttttggcct gtggagaaaa aaaatccatg ctcaaaggcc
      601 gtcaagtagc tgtgatgcag gggagacact cacttagcct cattaagcca aagctctctt
      661 ttgcttgtct tttgttctgc atgacagcag cctctttgtt gtataataat tcattccaag
      721 ttcgttccaa gctaccgaaa ctggtgaact gtggccattt taatgagaag tattggtggt
      781 atgcataatc tggaagccac gtgggccaga caatcacaga gcactgcagc cagcaaaatg
      841 agatattgtc gaaatcacaa atttgtcaag ttgtttttgg acagggtatt ataataagca
      901 ctaaaatatg catcctatta ctctgcatct gccagtcaaa gatcactgta atgaatgcgt
      961 gctgcagggt ataaccaggc cgttttccta agtaaaacaa accacagagt acagaaaaga
     1021 attgtgtggg tttttttttt tttttttggc attgtttgac tttttttttt ttcctaatcc
     1081 ttttgcacca ggagtcaggt tctcctggca tggtgtccac tgctagcagt gaatacacag
     1141 gccctgctgg tgccagggag ttaacatgaa tacattgctt ttttaatagg tgtgcttacc
     1201 atctgagaat acaaaacctc aaactcaagg cttcaattga gtctactgaa gaattcctgg
     1261 gagagaaaag ggaaaaagga ggagaaaaaa atatctctcc caatgatcct aagccttatg
     1321 ttttatcctt ttctacttca aatggcttag cctttgggac ttgttttgtt gtttgttttt
     1381 ctgtttgttt atttttgctt ttttgtctgt ctgttgattt atttgctttt tagatgaacc
     1441 taaagctttg ggaagaaatg ggtgagatgc tagagctcca ggaacatcca aactgagcag
     1501 ccggggtccc ccccaccccc caccccgccc cacgcggcaa ctttgagcct gtgctgggac
     1561 agagcctcta gttcctaaat tagtccatga ggtcagaggc agcactgcca ttgtaacgcg
     1621 attggagagg atcacgtcac cggacacgcc cccaggcatc tccctgggtc tcctaaactt
     1681 ggcggggaga agttttagcc cttaagtttt agcctttaac ccccatattc agaactgtgc
     1741 gagttggcga aaccccacaa atcacaacaa actgtacaca acaccgagct agaggtgatc
     1801 tttcttgtcc attccacaca ggccttagta atgcgtcgcc atagcaacag tgtcactagt
     1861 agcaccagca cttccccaca ccctccccct caggaatccg tactctccag tgaaccccag
     1921 aaacctctgg agagttctgg acaagggcgg aacccacaac tccgattact caagggaggc
     1981 ggggaagctc caccagacgc gaaactgctg gaagattcct ggccccaagg cctcctccgg
     2041 ctcgctgatt ggcccagcgg agagtgggcg gggccggtga agactcctta aaggcgcagg
     2101 gcggcgagca ggtcaccaga cgctgacagc tactcagaac caaatctggt tccatccaga
     2161 gacaagcgaa gacaagagaa gcagagcgag cggcgcgttc ccgatcctcg gccaggacca
     2221 gccttcccca gagcatccct gccgcggagc gcaaccttcc caggagcatc cctgccgcgg
     2281 agcgcaactt tccccggagc atccacgccg cggagcgcag ccttccagaa gcagagcgcg
     2341 gcgccatggc ggcgcgccga agttggtcgt gaggcactgg gcaggtaagt atcaaggtta
     2401 caagacaggt ttaaggagac caatagaaac tgggcttgtc gagacagaga agactcttgc
     2461 gtttctgata ggcacctatt ggtcttactg acatccactt tgcctttctc tccacaggtg
     2521 tccactccca ggcggccgcc accatgtcca acctgctgac tgtgcaccaa aacctgcctg
     2581 ccctccctgt ggatgccacc tctgatgaag tcaggaagaa cctgatggac atgttcaggg
     2641 acaggcaggc cttctctgaa cacacctgga agatgctcct gtctgtgtgc agatcctggg
     2701 ctgcctggtg caagctgaac aacaggaaat ggttccctgc tgaacctgag gatgtgaggg
     2761 actacctcct gtacctgcaa gccagaggcc tggctgtgaa gaccatccaa cagcacctgg
     2821 gccagctcaa catgctgcac aggagatctg gcctgcctcg cccttctgac tccaatgctg
     2881 tgtccctggt gatgaggaga atcagaaagg agaatgtgga tgctggggag agagccaagc
     2941 aggccctggc ctttgaacgc actgactttg accaagtcag atccctgatg gagaactctg
     3001 acagatgcca ggacatcagg aacctggcct tcctgggcat tgcctacaac accctgctgc
     3061 gcattgccga aattgccaga atcagagtga aggacatctc ccgcaccgat ggtgggagaa
     3121 tgctgatcca cattggcagg accaagaccc tggtgtccac agctggtgtg gagaaggccc
     3181 tgtccctggg ggttaccaag ctggtggaga gatggatctc tgtgtctggt gtggctgatg
     3241 accccaacaa ctacctgttc tgccgggtca gaaagaatgg tgtggctgcc ccttctgcca
     3301 cctcccaact gtccacccgg gccctggaag ggatctttga ggccacccac cgcctgatct
     3361 atggtgccaa ggatgactct gggcagagat acctggcctg gtctggccac tctgccagag
     3421 tgggtgctgc cagggacatg gccagggctg gtgtgtccat ccctgaaatc atgcaggctg
     3481 gtggctggac caatgtgaac atagtgatga actacatcag aaacctggac tctgagactg
     3541 gggccatggt gaggctgctc gaggatgggg actgagcggc cgcgatcgca gacatgataa
     3601 gatacattga tgagtttgga caaaccacaa ctagaatgca gtgaaaaaaa tgctttattt
     3661 gtgaaatttg tgatgctatt gctttatttg taaccattat aagctgcaat aaacaagtta
     3721 acaacaacaa ttgcattcat tttatgtttc aggttcaggg ggagatgtgg gaggtttttt
     3781 aaagcaagta aaacctctac aaatgtggta gtcgaaattc ccgataagga tcttcctaga
     3841 gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg aacccctagt
     3901 gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa
     3961 ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagcct
     4021 taattaacct aattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt
     4081 tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga
     4141 ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc
     4201 ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact
     4261 tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc
     4321 cggctttccc cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt
     4381 acggcacctc gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc
     4441 ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt
     4501 gttccaaact ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat
     4561 tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa
     4621 ttttaacaaa atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga
     4681 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
     4741 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
     4801 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
     4861 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
     4921 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
     4981 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
     5041 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
     5101 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
     5161 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
     5221 gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
     5281 aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg
     5341 ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
     5401 tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
     5461 tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
     5521 gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
     5581 atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
     5641 ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
     5701 aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
     5761 ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
     5821 ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
     5881 tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
     5941 cagataccaa atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct
     6001 gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
     6061 gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
     6121 tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
     6181 ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
     6241 gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
     6301 ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
     6361 tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
     6421 ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
     6481 gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
     6541 acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg
     6601 cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg
     6661 aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag
     6721 gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt
     6781 cacacaggaa acagctatga ccatgattac gccagattta attaagg
//
