LOCUS       sequence-73233-e        7111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with NOV (Ple303) promoter driving expression of iCre..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7111)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 7111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2025
FEATURES             Location/Qualifiers
     source          1..7111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     RBS             1985..1996
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     intron          2669..2801
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     2784..2803
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    3882..4003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3893..3912)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3947..3966
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4172..4301
                     /label=AAV2 ITR
     primer_bind     complement(4320..4337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4320..4336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4329..4351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4478..4933
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4565..4584)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4775..4796
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4959..5063
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5064..5132,5133..5924)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5282..5301)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6095..6683
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6584..6603
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    6971..6992
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7007..7012,7013..7030,7031..7037)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7045..7061
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7050..7072
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7069..7085
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7069..7085
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccgg gtgaactctg tggtgactgc tttaattaat
      241 ttatgtaatt ggaatttcac caagacataa atttactcat atctattttt aaagacagga
      301 atattgatct tggaccagca ataaattcct gtaatatttg tcctcagtaa aaagccaaga
      361 caattctagg ccatttcttt ttgtcttgaa aacgttatgc caaaatcatc attttgttgg
      421 ctgcatacat cctgatttat attcgatgag agttataaaa ggcttttata gtggagatgg
      481 ccgagtgaca ttttcctata aatcacgatc atttttactg ctacaaagtt ctaggactga
      541 acttcaaata caaccgtgct gtaaactctg tggatggaac cacatacttg ctgaatgaga
      601 aacatgtttc cctttcacca tgagtgactg catattccag tcagtctctg gcagatgaga
      661 gcatcactaa aacccaggga accatgctca gctttgatga agcagcttgt gaaacgcagg
      721 tcagaagaac ttaccatctt gggaaagtga taaagctgga tggtctctaa catttaccta
      781 aacttgaatt ggctattttt tgtacataca ttttccattt caactattag cttagaagtt
      841 tacaaaagaa cagtccagtt tttttttttc tattataaca gatacagcat taccatttgt
      901 aatcgttgat caaggcataa gcaatttctt gcttgtcaat ttgaacatct agccaggaat
      961 aaatagcaca cagtatgtgt aatgcaatct tcaaagcgtt ttattggaac agttcttaga
     1021 cctcatacaa ggaaataaaa aaataagaaa tgtaaatgaa tgtgatgtca gactgaggaa
     1081 gcaagtctct agagatccct ggaagttaga atggatttta aaattcatta tgattgaatt
     1141 ataaaataaa tgaaaaccag cagggttaaa tatgaggttg atgggagcaa gaaaagaaag
     1201 ttatttggat acccatggca gggagcatgc aagttcaggt tcacctctag ggcaaggaag
     1261 aattttcttc aaggttcttc tctttcagtg tttactctgt agcaggcact gtgactgtgt
     1321 aagtggcaca cccttaagat gcccccaagt tactttgccc gccttggtgg cccccatttg
     1381 gtcaccgggc tcactgcgtc ttctgtccca gctgagtggt ttctccttgt ctcgcctgcc
     1441 ttcagtgtta atttccagaa aaattgaaag aggaggggtt aagttaaaca tacactgtga
     1501 gttgttctta cgctacagtt gaagagagat cttgaaatat atttttcatt cattttctct
     1561 ctttgtgtct cccctatgtg agaaatatgg catgcacaat tggcgcctat attatcctct
     1621 agccaatact ttatcccagt tgccttagca actgtcaata tttgtctggt atgtcacagc
     1681 cccagttggc tgatggctgg ttgaacatct acttgtaaag tttaggaaag agagacaccc
     1741 agtggtctaa attaactgag ttgctgaaag cctgcttcat tttattttca cttcagaaat
     1801 gtttattaat ttttaaatct gatcttctgt taatcatcaa caatccccac aattaaactt
     1861 atgaagggag aaaagaacat gtgttggcac acttactttt tctgtaaagt cccagacagt
     1921 aaatatttta ggctttgcgg tccccctgcc actcccctcc ccccaacaca cacacacttt
     1981 tctctttctc ctctttctct cctttcctcc cttgcttctc tcccctccct ctcaacacat
     2041 tcaatgagtg ccctaaacgg tgacaaactt gcatgtgctt ccctcatgac taaacccctg
     2101 ggccttctgc caatcccctg cagatggccc caaactgcag gcatcccgta aggggacccc
     2161 acgcttgcag ccctggttgg aacggtcagg gtggaggagg atggtgggga gtggtggtgt
     2221 cttcgtcctg ggagaaggcg aagcaacttc caggaggaaa cgggcgtttc cttcccacgc
     2281 gctcgagcga gccctgggtc ctggcctcgg aactccaccc agcccctccc caccctctgg
     2341 gaaaagccag tcgccacaca caggcacacg caggccccgg cgccgcgccc taaggagagc
     2401 agcacccaca gccaattgcc atggcaaccc cggggttcgt tccacttccc cacccagccg
     2461 atctcccccc tcctccctgc actgcagcca accggcttgt gcgcgtccca ggagcgcgct
     2521 ataaaacctg tgctgggcgt gatcggcaag caccggacca gggggaaggc gagcagtgcc
     2581 aatctacagc gaagaaagtc tcgtttggta aaagcgagag gggaaagcct gagcggcgcg
     2641 ccgaagttgg tcgtgaggca ctgggcaggt aagtatcaag gttacaagac aggtttaagg
     2701 agaccaatag aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     2761 tattggtctt actgacatcc actttgcctt tctctccaca ggtgtccact cccaggcggc
     2821 cgccaccatg tccaacctgc tgactgtgca ccaaaacctg cctgccctcc ctgtggatgc
     2881 cacctctgat gaagtcagga agaacctgat ggacatgttc agggacaggc aggccttctc
     2941 tgaacacacc tggaagatgc tcctgtctgt gtgcagatcc tgggctgcct ggtgcaagct
     3001 gaacaacagg aaatggttcc ctgctgaacc tgaggatgtg agggactacc tcctgtacct
     3061 gcaagccaga ggcctggctg tgaagaccat ccaacagcac ctgggccagc tcaacatgct
     3121 gcacaggaga tctggcctgc ctcgcccttc tgactccaat gctgtgtccc tggtgatgag
     3181 gagaatcaga aaggagaatg tggatgctgg ggagagagcc aagcaggccc tggcctttga
     3241 acgcactgac tttgaccaag tcagatccct gatggagaac tctgacagat gccaggacat
     3301 caggaacctg gccttcctgg gcattgccta caacaccctg ctgcgcattg ccgaaattgc
     3361 cagaatcaga gtgaaggaca tctcccgcac cgatggtggg agaatgctga tccacattgg
     3421 caggaccaag accctggtgt ccacagctgg tgtggagaag gccctgtccc tgggggttac
     3481 caagctggtg gagagatgga tctctgtgtc tggtgtggct gatgacccca acaactacct
     3541 gttctgccgg gtcagaaaga atggtgtggc tgccccttct gccacctccc aactgtccac
     3601 ccgggccctg gaagggatct ttgaggccac ccaccgcctg atctatggtg ccaaggatga
     3661 ctctgggcag agatacctgg cctggtctgg ccactctgcc agagtgggtg ctgccaggga
     3721 catggccagg gctggtgtgt ccatccctga aatcatgcag gctggtggct ggaccaatgt
     3781 gaacatagtg atgaactaca tcagaaacct ggactctgag actggggcca tggtgaggct
     3841 gctcgaggat ggggactgag cggccgcgat cgcagacatg ataagataca ttgatgagtt
     3901 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     3961 tattgcttta tttgtaacca ttataagctg caataaacaa gttaacaaca acaattgcat
     4021 tcattttatg tttcaggttc agggggagat gtgggaggtt ttttaaagca agtaaaacct
     4081 ctacaaatgt ggtagtcgaa attcccgata aggatcttcc tagagcatgg ctacgtagat
     4141 aagtagcatg gcgggttaat cattaactac aaggaacccc tagtgatgga gttggccact
     4201 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4261 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gccttaatta acctaattca
     4321 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     4381 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     4441 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     4501 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     4561 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     4621 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     4681 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     4741 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     4801 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     4861 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     4921 acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
     4981 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
     5041 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
     5101 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
     5161 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
     5221 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
     5281 tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc ggtcgccgca
     5341 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
     5401 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
     5461 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
     5521 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
     5581 acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc aaactattaa
     5641 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
     5701 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
     5761 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
     5821 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
     5881 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
     5941 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
     6001 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     6061 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     6121 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     6181 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     6241 ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     6301 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     6361 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     6421 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     6481 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     6541 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     6601 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     6661 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     6721 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     6781 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     6841 agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc cccgcgcgtt
     6901 ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     6961 gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     7021 ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     7081 atgaccatga ttacgccaga tttaattaag g
//
