LOCUS       sequence-73238-e        7404 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with NR2E1 (Ple264) promoter driving expression of 
            EmGFP..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7404)
  AUTHORS   Korecki AJ, Hickmott JW, Lam SL, Dreolini L, Mathelier A, Baker O, 
            Kuehne C, Bonaguro RJ, Smith J, Tan CV, Zhou M, Goldowitz D, 
            Deussing JM, Stewart AF, Wasserman WW, Holt RA, Simpson EM
  TITLE     Twenty-Seven Tamoxifen-Inducible iCre-Driver Mouse Strains for Eye 
            and Brain; Including Seventeen Carrying a New Inducible-First 
            Constitutive-Ready Allele.
  JOURNAL   Genetics. 2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984.
  PUBMED    30765420
REFERENCE   2  (bases 1 to 7404)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Genetics. 
            2019 Feb 14. pii: genetics.119.301984. doi: 
            10.1534/genetics.119.301984."
COMMENT     Sequence Label: pEMS2112
FEATURES             Location/Qualifiers
     source          1..7404
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     intron          3274..3406
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     3389..3408
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      3427..3436
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3433..3435,3436..3438,3439..4152)
                     /codon_start=1
                     /product="Emerald GFP"
                     /label=EmGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHKVYITADKQKNGIK
                     VNFKTRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3478..3499)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3739..3758)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4086..4107
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    4175..4296
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4186..4205)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     4240..4259
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   4465..4594
                     /label=AAV2 ITR
     primer_bind     complement(4613..4630)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4613..4629)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4622..4644)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4771..5226
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4858..4877)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5068..5089
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5252..5356
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5357..5425,5426..6217)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5575..5594)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6388..6976
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6877..6896
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    7264..7285
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7300..7305,7306..7323,7324..7330)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7338..7354
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7343..7365
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7362..7378
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7362..7378
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccac gcgtgttgcc accggatgaa aggtggagga
      241 ggcagaaacc taaacaagtg aattcaatta catctgaaaa agattaggct aaattaggat
      301 tcattccttg ggccagttcc aggaaatatt tgtgaaaagt tggttgaaag aatactacca
      361 tctaaaccct aattttgcta ttacgtaaaa gccccgattc ctttgcagta aaacattaga
      421 aaatgttttc ctcattttgt ttttgctact ggtgtgtgac ctcttaattt aattttggtt
      481 tttgctcata taaatcaatg gccgtggaaa ctttttcctg tggtgcagca aagacagggg
      541 aaaagtagaa catcagtaat tatattttag gcacttaatt tatttaatga gcagttatca
      601 agcaccctta atggttccag ccataaacta taggctcctc ccgctccctc ggcctccagg
      661 ttcaataagg attacatcat ggagcaagta tgcggggcag cctgagaatc aaagccgtca
      721 gcaccggcgg ctggttaact gtactgttgc tgaaacacat ccttcccacc ccctccccgc
      781 gcccctctcc ctgccccggc cctctgcttt tcgtaccatt aaggatagca ttattgggga
      841 gaggaaaatc tgatcaaaac tgtcgaccgc caacgacaga ttttctatgg attctataaa
      901 ggccacctta acgatttccc taattaatgg atgacatctt tggctgaagg agaaacgatg
      961 gattcgactc tgtcttcgtc tttgttaacc ttctaattaa ctttcgtagg tcacccaggt
     1021 ctcccaaacc tgaaaagtga agaggcccat tgtgcgagga gcagtaaact tactttttct
     1081 gctggggtac ttaaaaggga gagcggctat aggccgagaa aaataactcc atttaaaagc
     1141 tttgtggctg cagagaatac ataggtccct cccttagaaa ataattgaaa caccttttat
     1201 accaatattt ctatgcataa taaaaacaaa ttagcaaaat gcatctaaat tcagcagcac
     1261 tttcaaatta ccttttttgg aatgtaagcc tctgcaagtc aacaaaagga gttgggttta
     1321 gagagaaaat tgcaactgca acagctatca gcctttatgc tgagagagca agttacacct
     1381 gaaattttag aggctaagga gaaatgtctg caacctacct cctcagttgt gaactcctgt
     1441 acctgaaagg ccaaatcctc ctcctcccag caggcaatca tcttatgccc tgaagcatga
     1501 gatctgatta cccctatcat catcttagct tgcatagata cagatgttat taacggctgt
     1561 agtttatcca accctttttt aaaacgcagc tgcagcattt gacttgggga cctcctgtga
     1621 atcccactgg ttgactccgt cttgcacaaa gcacgtgggg tttgggggag ggagaagaga
     1681 gagggaggtt tgttttgttt tggagggttt tttttttttt tttttgaatc agagttccct
     1741 aaatagccac tcggcatctt tgtaagtgat gtgaattctg aaattactta ctagaatagg
     1801 acaacagagt actgtgtgaa ctagatctgt tggaaacaaa gcatagtaga atgtatgatg
     1861 cttttcactt cattggcaga gatcaaatat gtggtcctga tatactcatc cacataataa
     1921 agaattgtag cttattctga aatatattgg ccacattcaa actggagtac ctggttttac
     1981 ataacaattg tttctacaga agaccccatc ctcagccctg actttccgta gatatgtgca
     2041 aaatgagtaa attactcacc tcgggccaga tccaagtttt acccaacaga aggggcaccg
     2101 gaccaagaat gaaccaactc acatggccat gtccggcgcg cacaatcaca cgccagcaca
     2161 cagccaccca atttcttccg cgaatctatc tggcactctg gagagagggg gaaaagcgtt
     2221 ttgagaaagc cccgtcaccc ctccccttcc ttcttgccgt gaaatatacg aattcatttt
     2281 tattacgagc cgcaccgtcc tcaccatcac gcacgcacag agccacactc ccatattcac
     2341 actttctaac tcgtaagctc cgacagcgcc tgcattttct ttgggagccg cttggaggtt
     2401 cattaatatc attagcattt aaccccctcc ctcttcccat cccctccccg cacatggctg
     2461 acgtcagacc ccgccaggag ttgggggaaa agctaagtgg gccagggacg ccctattccc
     2521 ctccccgcgg ctgcctgtca gagcgcttct ggagatatta caggggaccc agcccgcagc
     2581 gacaggcaca aagtcacggg gtaatgaact tcggggaccc ttcgccgctg cgtgcgcggc
     2641 tctccccgga aacccggacc tggccgcctc ttccctcgga agatttccca gcaatctagt
     2701 tttcccactc tgcgcttggg ttccggcagc gcggagcccg tctgcctctg agactgcggt
     2761 agtgttttcc ttctttcctt gggagaccag cggtcggcag agattgccca cactctgcat
     2821 gcctatgtag agggagagat cgaagactga gtgacaggaa tggggaaaaa gagggatttc
     2881 gctccgtagg aaggccattt tcgtgtctcc atctctgtct ttcaacatcc ctctcttgct
     2941 gttcttcctt cttcctcagt cttcctgtcc atctctccat ctgtctgtcc atgtgtgtgt
     3001 ccatatcaag cagcattccc agcagctgcg gttttgcaag agccgggaag aaacttaagg
     3061 atgcttaaat ttccactgtt ggacgaattc tgagcgccca gggagcagcg cagcgcgcga
     3121 ctgacaccca cctgtcccgc ccaggagcct tgcaggctgg agggcagctg gagagcggcg
     3181 gcgcccggcg gcgaggcggg cgctgccggc cgggactcgg gcagcgccca ccaaccgctg
     3241 gcgcgccgaa gttggtcgtg aggcactggg caggtaagta tcaaggttac aagacaggtt
     3301 taaggagacc aatagaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     3361 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtgt ccactcccag
     3421 gcggccgcca ccatggtgag caagggcgag gagctgttca ccggggtggt gcccatcctg
     3481 gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga gggcgagggc
     3541 gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa gctgcccgtg
     3601 ccctggccca ccctcgtgac caccttcacc tacggcgtgc agtgcttcgc ccgctacccc
     3661 gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta cgtccaggag
     3721 cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt gaagttcgag
     3781 ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga ggacggcaac
     3841 atcctggggc acaagctgga gtacaactac aacagccaca aggtctatat caccgccgac
     3901 aagcagaaga acggcatcaa ggtgaacttc aagacccgcc acaacatcga ggacggcagc
     3961 gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc cgtgctgctg
     4021 cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa cgagaagcgc
     4081 gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg catggacgag
     4141 ctgtacaagt aagcggccgc gatcgcagac atgataagat acattgatga gtttggacaa
     4201 accacaacta gaatgcagtg aaaaaaatgc tttatttgtg aaatttgtga tgctattgct
     4261 ttatttgtaa ccattataag ctgcaataaa caagttaaca acaacaattg cattcatttt
     4321 atgtttcagg ttcaggggga gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa
     4381 tgtggtagtc gaaattcccg ataaggatct tcctagagca tggctacgta gataagtagc
     4441 atggcgggtt aatcattaac tacaaggaac ccctagtgat ggagttggcc actccctctc
     4501 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
     4561 cccgggcggc ctcagtgagc gagcgagcgc gcagccttaa ttaacctaat tcactggccg
     4621 tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag
     4681 cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc
     4741 aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg
     4801 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc
     4861 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4921 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4981 ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     5041 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     5101 accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg gcctattggt
     5161 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgctta
     5221 caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta
     5281 aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata
     5341 ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc
     5401 ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga
     5461 agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct
     5521 tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg
     5581 tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta
     5641 ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat
     5701 gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt
     5761 acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga
     5821 tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga
     5881 gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga
     5941 actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc
     6001 aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc
     6061 cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg
     6121 tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat
     6181 cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata
     6241 tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct
     6301 ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga
     6361 ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg
     6421 cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc
     6481 aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct
     6541 agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc
     6601 tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt
     6661 ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg
     6721 cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct
     6781 atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag
     6841 ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag
     6901 tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg
     6961 gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg
     7021 gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac
     7081 cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt
     7141 gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat
     7201 tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc
     7261 aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta tgcttccggc
     7321 tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca gctatgacca
     7381 tgattacgcc agatttaatt aagg
//
