LOCUS       sequence-73243-e         716 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial S. pyogenes Cas9 (SP) + tracrRNA expression, 
            cloDF13/spectinomycin.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-SPcas
FEATURES             Location/Qualifiers
     source          1..716
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      313..340
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agatcgcttg gcctcgcgcg cagatcagtt ggaagaattt gtccactacg tgaaaggcga
       61 gatcaccaag gtagtcggca aataatgtct aacaattcgt tcaagccgag gggccgcaag
      121 atccggccac gatgacccgg tcgtcggttc agggcagggt accaggcacg cctaaccgtc
      181 agtgagattg gatgagtgaa cgatattgat cgagaagagc cctgcgcagc cgctgccgtg
      241 cctgcaggaa gcaacggccc ggagggtggc gggcaggacg cccgccataa actgccaggc
      301 atcaaattaa gcagaaggcc atcctgacgg atggcctttt tgcgtttcta caaactctgc
      361 tagcttctag agcacagcta acaccacgtc gtccctatct gctgccctag gtctatgagt
      421 ggttgctgga taactttacg ggcatgcata aggctcgtat gatatattca gggagaccac
      481 aacggtttcc ctctacaaat aattttgttt aacttttact agaggaggag gcaaaaatgg
      541 ataagaaata ctcaataggc ttagatatcg gcacaaatag cgtcggatgg gcggtgatca
      601 ctgatgaata taaggttccg tctaaaaagt tcaaggttct gggaaataca gaccgccaca
      661 gtatcaaaaa aaatcttata ggggctcttt tatttgacag tggagagaca gcggaa
//
