LOCUS       sequence-73244-e        5633 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  AAV plasmid with smCBA promoter driving expression of iCre. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5633)
  AUTHORS   Simpson E, Korecki AJ, Fornes O, McGill TJ, Cueva-Vargas JL, 
            Agostinone J, Farkas RA, Hickmott JW, Lam SL, Mathelier A, Renner 
            LM, Stoddard J, Zhou M, Di Polo A, Neuringer M, Wasserman WW
  TITLE     New MiniPromoter Ple345 (NEFL) Drives Strong and Specific Expression
            in Retinal Ganglion Cells of Mouse and Primate Retina.
  JOURNAL   Hum Gene Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118.
  PUBMED    30062914
REFERENCE   2  (bases 1 to 5633)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Hum Gene 
            Ther. 2018 Jul 31. doi: 10.1089/hum.2018.118."
COMMENT     Sequence Label: pEMS2117
FEATURES             Location/Qualifiers
     source          1..5633
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
     enhancer        276..579
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        581..858
                     /label=chicken beta-actin promoter
     primer_bind     1115..1134
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          1190..1322
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1305..1324
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     polyA_signal    2403..2524
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2414..2433)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2468..2487
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     repeat_region   2694..2823
                     /label=AAV2 ITR
     primer_bind     complement(2842..2859)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2842..2858)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2851..2873)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3000..3455
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3087..3106)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3297..3318
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        3481..3585
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3586..3654,3655..4446)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3804..3823)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4617..5205
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5106..5125
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     protein_bind    5493..5514
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(5529..5534,5535..5552,5553..5559)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5567..5583
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     5572..5594
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     5591..5607
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     5591..5607
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct tgtagttaat gattaacccg ccatgctact tatctacgta gccatgctct
      181 aggaagatcg gaattcccta ggccggccgg taccctagtt attaatagta atcaattacg
      241 gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac ggtaaatggc
      301 ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc
      361 atagtaacgc caatagggac tttccattga cgtcaatggg tggactattt acggtaaact
      421 gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat
      481 gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact
      541 tggcagtaca tctacgtatt agtcatcgct attaccatgg tcgaggtgag ccccacgttc
      601 tgcttcactc tccccatctc ccccccctcc ccacccccaa ttttgtattt atttattttt
      661 taattatttt gtgcagcgat gggggcgggg gggggggggg ggcgcgcgcc aggcggggcg
      721 gggcggggcg aggggcgggg cggggcgagg cggagaggtg cggcggcagc caatcagagc
      781 ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc ggcggcggcc ctataaaaag
      841 cgaagcgcgc ggcgggcggg agtcgctgcg acgctgcctt cgccccgtgc cccgctccgc
      901 cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac aggtgagcgg
      961 gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac ggcttgtttc
     1021 ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ctagagcctc tgctaaccat
     1081 gttcatgcct tcttcttttt cctacagctc ctgggcaacg tgctggttat tgtgctgtct
     1141 catcattttg gcaaaggcgc gccgaagttg gtcgtgaggc actgggcagg taagtatcaa
     1201 ggttacaaga caggtttaag gagaccaata gaaactgggc ttgtcgagac agagaagact
     1261 cttgcgtttc tgataggcac ctattggtct tactgacatc cactttgcct ttctctccac
     1321 aggtgtccac tcccaggcgg ccgccaccat gtccaacctg ctgactgtgc accaaaacct
     1381 gcctgccctc cctgtggatg ccacctctga tgaagtcagg aagaacctga tggacatgtt
     1441 cagggacagg caggccttct ctgaacacac ctggaagatg ctcctgtctg tgtgcagatc
     1501 ctgggctgcc tggtgcaagc tgaacaacag gaaatggttc cctgctgaac ctgaggatgt
     1561 gagggactac ctcctgtacc tgcaagccag aggcctggct gtgaagacca tccaacagca
     1621 cctgggccag ctcaacatgc tgcacaggag atctggcctg cctcgccctt ctgactccaa
     1681 tgctgtgtcc ctggtgatga ggagaatcag aaaggagaat gtggatgctg gggagagagc
     1741 caagcaggcc ctggcctttg aacgcactga ctttgaccaa gtcagatccc tgatggagaa
     1801 ctctgacaga tgccaggaca tcaggaacct ggccttcctg ggcattgcct acaacaccct
     1861 gctgcgcatt gccgaaattg ccagaatcag agtgaaggac atctcccgca ccgatggtgg
     1921 gagaatgctg atccacattg gcaggaccaa gaccctggtg tccacagctg gtgtggagaa
     1981 ggccctgtcc ctgggggtta ccaagctggt ggagagatgg atctctgtgt ctggtgtggc
     2041 tgatgacccc aacaactacc tgttctgccg ggtcagaaag aatggtgtgg ctgccccttc
     2101 tgccacctcc caactgtcca cccgggccct ggaagggatc tttgaggcca cccaccgcct
     2161 gatctatggt gccaaggatg actctgggca gagatacctg gcctggtctg gccactctgc
     2221 cagagtgggt gctgccaggg acatggccag ggctggtgtg tccatccctg aaatcatgca
     2281 ggctggtggc tggaccaatg tgaacatagt gatgaactac atcagaaacc tggactctga
     2341 gactggggcc atggtgaggc tgctcgagga tggggactga gcggccgcga tcgcagacat
     2401 gataagatac attgatgagt ttggacaaac cacaactaga atgcagtgaa aaaaatgctt
     2461 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     2521 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     2581 tttttaaagc aagtaaaacc tctacaaatg tggtaggtcg aaattcccga taaggatctt
     2641 cctagagcat ggctacgtag ataagtagca tggcgggtta atcattaact acaaggaacc
     2701 cctagtgatg gagttggcca ctccctctct gcgcgctcgc tcgctcactg aggccgggcg
     2761 accaaaggtc gcccgacgcc cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg
     2821 cagccttaat taacctaatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     2881 tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     2941 cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga
     3001 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
     3061 tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
     3121 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
     3181 tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc
     3241 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
     3301 actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata
     3361 agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa
     3421 cgcgaatttt aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg
     3481 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     3541 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     3601 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     3661 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     3721 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     3781 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     3841 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     3901 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     3961 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     4021 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     4081 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     4141 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     4201 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     4261 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     4321 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     4381 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     4441 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     4501 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     4561 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     4621 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     4681 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     4741 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     4801 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     4861 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     4921 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     4981 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     5041 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     5101 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     5161 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     5221 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     5281 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     5341 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     5401 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     5461 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     5521 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     5581 caatttcaca caggaaacag ctatgaccat gattacgcca gatttaatta agg
//
