LOCUS       sequence-73248-e         789 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial nuclease-null ST1 Cas9 expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 789)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 789)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: DS-ST1casN-
FEATURES             Location/Qualifiers
     source          1..789
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      319..346
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
       61 aggcgagatc accaaggtag tcggcaaata atgtctaaca attcgttcaa gccgaggggc
      121 cgcaagatcc ggccacgatg acccggtcgt cggttcaggg cagggtacca ggcacgccta
      181 accgtcagtg agattggatg agtgaacgat attgatcgag aagagccctg cgcagccgct
      241 gccgtgcctg caggaagcaa cggcccggag ggtggcgggc aggacgcccg ccataaactg
      301 ccaggcatca aattaagcag aaggccatcc tgacggatgg cctttttgcg tttctacaaa
      361 ctctgctagc ttctagagca cagctaacac cacgtcgtcc ctatctgctg ccctaggtct
      421 atgagtggtt gctggataac tttacgggca tgcataaggc tcgtatgata tattcaggga
      481 gaccacaacg gtttccctct acaaataatt ttgtttaact tttactagag gaggaggcaa
      541 aaatgagcga cctggtgctg ggcctggcca tcggcatcgg cagcgtgggc gtgggcatcc
      601 tgaacaaggt gaccggcgag atcatccaca agaacagtcg catcttccct gctgctcagg
      661 ctgagaacaa cctggtgcgc cgcaccaacc gccagggtcg ccggcttgct cgccgcaaga
      721 agcaccggcg cgtgcgcctg aaccgcctgt tcgaggagag cggcctgatc accgacttca
      781 ccaagatca
//
