LOCUS       sequence-73250-e         979 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial SP Cas9 targeting filamentous phage gene III at five 
            protospacers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 836 to 870)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 979)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: EE-SP!gIII
FEATURES             Location/Qualifiers
     source          1..979
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(222..308)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     repeat_region   465..500
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   531..566
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   597..632
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   663..698
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   729..764
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   795..830
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     promoter        complement(836..870)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
ORIGIN
        1 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
       61 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      121 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
      181 accgctcgcc gcagccgaac gccctaggtc tagggcggcg gatttgtcct actcaggaga
      241 gcgttcaccg acaaacaaca gataaaacga aaggcccagt ctttcgactg agcctttcgt
      301 tttatttgat gcctctagca cgcgggacaa atccgccggg ctgcaggaat tcgatatcaa
      361 gcttttactt gtacagctcg tccatgcggg cttgtctgca ggatgctcta cgtgggtgcg
      421 gccgcaataa aaaccggcag gatgccggtt tttatggtgg gtgggttttg ggaccattca
      481 aaacagcata gctctaaaac gccttgctaa tggtaatggt gctactcgag gttttgggac
      541 cattcaaaac agcatagctc taaaacaaaa tgccgatgaa aacgcgctac agtctggttt
      601 tgggaccatt caaaacagca tagctctaaa acgtgatttt gattatgaaa agatggggat
      661 ccgttttggg accattcaaa acagcatagc tctaaaacat tgggcttgct atccctgaaa
      721 atgagggtgt tttgggacca ttcaaaacag catagctcta aaactacaga aaattcattt
      781 actaacgtaa gcttgttttg ggaccattca aaacagcata gctctaaaac attaagctag
      841 cactgtacct aggactgagc tagccgtcaa ggcgcgccat gactaacgtt ttcattgtcg
      901 ctagatggca gcgcctaaat gtagaatgat aaaaggatta agagattaat ttccctaaaa
      961 atgataaaac aagcgtttt
//
