LOCUS       sequence-73253-e        1009 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial SP Cas9 targeting filamentous phage gene III at five 
            protospacers.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 622 to 656)
  AUTHORS   Esvelt KM, Mali P, Braff JL, Moosburner M, Yaung SJ, Church GM
  TITLE     Orthogonal Cas9 proteins for RNA-guided gene regulation and editing.
  JOURNAL   Nat Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681.
  PUBMED    24076762
REFERENCE   2  (bases 1 to 1009)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2013 Sep 29. doi: 10.1038/nmeth.2681."
COMMENT     Sequence Label: EE-SP!gIII
FEATURES             Location/Qualifiers
     source          1..1009
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(8..94)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     repeat_region   251..286
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   317..352
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   383..418
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   449..484
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   515..550
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     repeat_region   581..616
                     /label=DR
                     /note="direct repeat for the Streptococcus pyogenes 
                     CRISPR/Cas system"
     promoter        complement(622..656)
                     /label=﻿BBa_J23100
                     /note="Anderson constitutive promoter 
                     (https://parts.igem.org/Promoters/Catalog/Anderson). 
                     Measured strength relative to BBa_J223100: 1.0
                     "
                     /citation=[1]
     misc_RNA        complement(821..899)
                     /label=tracrRNA
                     /note="trans-activating CRISPR RNA for the Streptococcus 
                     pyogenes CRISPR/Cas9 system"
ORIGIN
        1 gcggcggatt tgtcctactc aggagagcgt tcaccgacaa acaacagata aaacgaaagg
       61 cccagtcttt cgactgagcc tttcgtttta tttgatgcct ctagcacgcg ggacaaatcc
      121 gccgggctgc aggaattcga tatcaagctt ttacttgtac agctcgtcca tgcgggcttg
      181 tctgcaggat gctctacgtg ggtgcggccg caataaaaac cggcaggatg ccggttttta
      241 tggtgggtgg gttttgggac cattcaaaac agcatagctc taaaacgcct tgctaatggt
      301 aatggtgcta ctcgaggttt tgggaccatt caaaacagca tagctctaaa acaaaatgcc
      361 gatgaaaacg cgctacagtc tggttttggg accattcaaa acagcatagc tctaaaacgt
      421 gattttgatt atgaaaagat ggggatccgt tttgggacca ttcaaaacag catagctcta
      481 aaacattggg cttgctatcc ctgaaaatga gggtgttttg ggaccattca aaacagcata
      541 gctctaaaac tacagaaaat tcatttacta acgtaagctt gttttgggac cattcaaaac
      601 agcatagctc taaaacatta agctagcact gtacctagga ctgagctagc cgtcaaggcg
      661 cgccatgact aacgttttca ttgtcgctag atggcagcgc ctaaatgtag aatgataaaa
      721 ggattaagag attaatttcc ctaaaaatga taaaacaagc gttttgaaag cgcttgtttt
      781 tttggtttgc agtcagagta gaatagaagt atcaaaaaaa gcaccgactc ggtgccactt
      841 tttcaagttg ataacggact agccttattt taacttgcta tgctgttttg aatggttcca
      901 acaagattat tttataacat ttataacaaa taatcaagga gaaattcaaa gaaatttatc
      961 agccggtcag tcacctccta gctgactcaa atcaatgcgt gtttcataa
//
