LOCUS       sequence-73255-e         665 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 665)
  AUTHORS   Qi Y, Katagiri F
  TITLE     Purification of low-abundance Arabidopsis plasma-membrane protein 
            complexes and identification of candidate components.
  JOURNAL   Plant J. 2009 Mar;57(5):932-44. Epub 2008 Dec 4.
  PUBMED    19000159
REFERENCE   2  (bases 1 to 665)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J."; 
            date: "2009-03"; volume: "57(5)"; pages: "932-44. Epub 2008 Dec 4"
COMMENT     Sequence Label: pMDC32-HPB
FEATURES             Location/Qualifiers
     source          1..665
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(37..43,44..61,62..67))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(92..216)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        complement(276..620)
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     complement(312..332)
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
ORIGIN
        1 ctgaaaatct cgacggatcc taactcaaaa tccacacatt atacgagccg gaagcataaa
       61 gtgtaaagcc tggggtgcct aatgcggccg ccatagtgac tggatatgtt gtgttttaca
      121 gtattatgta gtctgttttt tatgcaaaat ctaatttaat atattgatat ttatatcatt
      181 ttacgtttct cgttcagctt ttttgtacaa acttgtttga tagcttggcg cgcctcgagg
      241 gggggcccgg tacccgggga tcctctagag tcgaggtcct ctccaaatga aatgaacttc
      301 cttatataga ggaagggtct tgcgaaggat agtgggattg tgcgtcatcc cttacgtcag
      361 tggagatatc acatcaatcc acttgctttg aagacgtggt tggaacgtct tctttttcca
      421 cgatgctcct cgtgggtggg ggtccatctt tgggaccact gtcggcagag gcatcttcaa
      481 cgatggcctt tcctttatcg caatgatggc atttgtagga gccaccttcc ttttccacta
      541 tcttcacaat aaagtgacag atagctgggc aatggaatcc gaggaggttt ccggatatta
      601 ccctttgttg aaaagtctca attgcccttt ggtcttctga gactgtatct ttgatatttt
      661 tggag
//
