LOCUS       sequence-73258-e         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning mammalian cell line expression vector with CMV promoter 
            and N-terminal streptavidin binding peptide, Myc tag and 3C protease
            cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pcDXNSM3
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..268)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(287..307)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     complement(398..415)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    404..628
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(761..780)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcagc ttttttgtac aaacttgtga tatcgaattc gctagctacc tttctcttct
      301 tttttggatc tacctttctc ttcttttttg gggatccact agttcagctc ttctgcataa
      361 taactatcta gagggcccgt ttaaacccgc tgatcagcct cgactgtgcc ttctagttgc
      421 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
      481 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
      541 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
      601 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag ctggggctct
      661 agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
      721 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
      781 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
      841 gggttccga
//
