LOCUS       sequence-73264-e        7294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  luciferase reporter with mouse Bmp2 promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7294)
  AUTHORS   Abrams KL, Xu J, Nativelle-Serpentini C, Dabirshahsahebi S, Rogers 
            MB
  TITLE     An evolutionary and molecular analysis of Bmp2 expression.
  JOURNAL   J Biol Chem. 2004 Apr 16;279(16):15916-28. Epub 2004 Feb 2.
  PUBMED    14757762
REFERENCE   2  (bases 1 to 7294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2004-04-16"; volume: "279(16)"; pages: "15916-28. 
            Epub 2004 Feb 2"
COMMENT     Sequence Label: pGL1.7XX
FEATURES             Location/Qualifiers
     source          1..7294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             1772..3424
                     /codon_start=1
                     /gene="luc"
                     /product="firefly luciferase"
                     /label=luciferase
                     /translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
                     HIEVNITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
                     ANDIYNERELLNSMNISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
                     MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
                     RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
                     IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
                     GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
                     GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
                     LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
                     FVDEVPKGLTGKLDARKIREILIKAKKGGKSKL"
     primer_bind     complement(1838..1856)
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     intron          3665..3730
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     CDS             3860..3880
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     polyA_signal    4305..4439
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4581..4598)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4752..5340)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4832..4851)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5511..6302,6303..6371))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6134..6153
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6372..6476)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      6503..6958
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6590..6609)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6800..6821
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     polyA_signal    7148..7269
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
ORIGIN
        1 cccgggaggt accgagctct tacgcgtgct agaaacagcc agggcctaat ttccctctgt
       61 gggttgctaa tccgatttag gtgaacgaac ctagagttat tttagctccc cgactgaaaa
      121 gctagcacac gtgggtaaaa aaatcattaa agcccctgct tctggtcttt ctcggtcttt
      181 gctttgcaaa ctggaaagat ctggttcaca acgtaacgtt attcactctg gtcttctaca
      241 ggaatgctca gcccatagtt ttgggggtcc tgtgggtagc cagtggtggt actatgaagg
      301 ctcctgaatg tagggagaaa tggaaagatt tcaaaaaaga atcctggctc agcagctttg
      361 gggacatttc cagctgagga agaaaactgg cttggccaca gccagagcct tactgctgga
      421 gacccagtgg agagagagga ccaggcagaa aattcaaagg tctcaaaccg gaattgtctt
      481 gttacctgac tctggagtag gtgggtgtgg aagggaagat aaatatcaca agtatcgaag
      541 tgatcgcttc tataaagaga atttctatta actctcattg tcccctcaca tggacacaca
      601 cacacacaca cacacacaca cacacacatc actagaaggg atgtccactt tacaagtgtg
      661 tatctatgtt cagaaacctg tacccgtatt tttataattt acataaataa atacatataa
      721 aatatatgca tctttttatt agattcattt atttgaatat aaatgtatga atatttataa
      781 aatgtaataa tgcactcaga tgtgtatcgg ctatttctcg acattttctt ctcaccattc
      841 aaaacagaag cgtttgctca catttttgcc aaaatgtcta ataacttgta agttctgttc
      901 ttctttttaa tgtgctctta cctaaaaact tcaaactcaa gttgaatatt ggcccaatga
      961 gggaactcag aggccagtgg actctggatt tgccctagtc tcccgcagct gtgggcgcgg
     1021 atccaggtcc cgggggtcgg cttcacactc atccgggacg cgacccctta gcggccgcgc
     1081 gctcgccccg ccccgctcca ccgcggccgc cccgtagggc gcgccgtcca cacccctgcg
     1141 cgccgctccc gcccgcccgg ggatccccgg cgcgctgcgc ctccgagggg gaggtgttcg
     1201 gccacggccg ggagggagcc ggcaggcggc gtctccttta aaagccgcga gcgccgcgcc
     1261 acggcgcctc cgtcgtcgcc gccggagtcc tcgccctgcc gcgcagagcc ctgctcgcac
     1321 tgcgcccgcc gccgctgcgc ttcccacagc ccgcccggga ttggcagccc cggacgtagc
     1381 ctccccaggc gacaccaggc accggacgcc ctcccggcga aagacgcgag ggtcacccgc
     1441 ggcttcgagg gactggcacg acacgggttg gaactccaga ctgtgcgcgc ctggcgctgt
     1501 ggcctcggct gtccgggaga agctagagtc gcggaccgac gctaagaacc gggagtccgg
     1561 agcacagtct taccctcaat gcggggccac tctgacccag gagtgagcgc ccaaggcgag
     1621 cgggcggaag agtgagtgga ccccaggctg ccacaaaaga cacttggccc gagggctcgg
     1681 agcgcgaggt cacccggttt ggcaacccga gacgcgcggc tggactgtct cgagatctaa
     1741 gtaagcttgg cattccggta ctgttggtaa aatggaagac gccaaaaaca taaagaaagg
     1801 cccggcgcca ttctatcctc tagaggatgg aaccgctgga gagcaactgc ataaggctat
     1861 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgaa
     1921 catcacgtac gcggaatact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1981 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     2041 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     2101 tgaacgtgaa ttgctcaaca gtatgaacat ttcgcagcct accgtagtgt ttgtttccaa
     2161 aaaggggttg caaaaaattt tgaacgtgca aaaaaaatta ccaataatcc agaaaattat
     2221 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     2281 tcatctacct cccggtttta atgaatacga ttttgtacca gagtcctttg atcgtgacaa
     2341 aacaattgca ctgataatga attcctctgg atctactggg ttacctaagg gtgtggccct
     2401 tccgcataga actgcctgcg tcagattctc gcatgccaga gatcctattt ttggcaatca
     2461 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     2521 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     2581 agagctgttt ttacgatccc ttcaggatta caaaattcaa agtgcgttgc tagtaccaac
     2641 cctattttca ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     2701 cgaaattgct tctgggggcg cacctctttc gaaagaagtc ggggaagcgg ttgcaaaacg
     2761 cttccatctt ccagggatac gacaaggata tgggctcact gagactacat cagctattct
     2821 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     2881 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaga gaggcgaatt
     2941 atgtgtcaga ggacctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     3001 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     3061 acacttcttc atagttgacc gcttgaagtc tttaattaaa tacaaaggat atcaggtggc
     3121 ccccgctgaa ttggaatcga tattgttaca acaccccaac atcttcgacg cgggcgtggc
     3181 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     3241 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     3301 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     3361 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agtccaaatt
     3421 gtaaaatgta actgtattca gcgatgacga aattcttagc tattgtaata ctgcgatgag
     3481 tggcagggcg gggcgtaatt tttttaaggc agttattggt gcccttaaac gcctggttgc
     3541 tacgcctgaa taagtgataa taagcggatg aatggcagaa attcgccgga tctttgtgaa
     3601 ggaaccttac ttctgtggtg tgacataatt ggacaaacta cctacagaga tttaaagctc
     3661 taaggtaaat ataaaatttt taagtgtata atgtgttaaa ctactgattc taattgtttg
     3721 tgtattttag attccaacct atggaactga tgaatgggag cagtggtgga atgcctttaa
     3781 tgaggaaaac ctgttttgct cagaagaaat gccatctagt gatgatgagg ctactgctga
     3841 ctctcaacat tctactcctc caaaaaagaa gagaaaggta gaagacccca aggactttcc
     3901 ttcagaattg ctaagttttt tgagtcatgc tgtgtttagt aatagaactc ttgcttgctt
     3961 tgctatttac accacaaagg aaaaagctgc actgctatac aagaaaatta tggaaaaata
     4021 ttctgtaacc tttataagta ggcataacag ttataatcat aacatactgt tttttcttac
     4081 tccacacagg catagagtgt ctgctattaa taactatgct caaaaattgt gtacctttag
     4141 ctttttaatt tgtaaagggg ttaataagga atatttgatg tatagtgcct tgactagaga
     4201 tcataatcag ccataccaca tttgtagagg ttttacttgc tttaaaaaac ctcccacacc
     4261 tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg tttattgcag
     4321 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
     4381 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggatcc
     4441 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     4501 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     4561 cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     4621 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     4681 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     4741 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     4801 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     4861 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     4921 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
     4981 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     5041 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     5101 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     5161 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
     5221 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     5281 ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     5341 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     5401 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     5461 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     5521 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     5581 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     5641 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     5701 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     5761 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     5821 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     5881 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     5941 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     6001 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     6061 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     6121 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     6181 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     6241 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     6301 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     6361 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     6421 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     6481 tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     6541 gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     6601 ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     6661 ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     6721 ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     6781 gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     6841 tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     6901 gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct tacaatttgc
     6961 cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     7021 ttacgccagc ccaagctacc atgataagta agtaatatta aggtacgtgg aggttttact
     7081 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     7141 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     7201 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     7261 tgtatcttat ggtactgtaa ctgagctaac ataa
//
