LOCUS       sequence-73278-e        1055 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            (c-terminal histag) by homologous recombination and selection for 
            phleomycin resistance..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1055)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 1055)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH3
FEATURES             Location/Qualifiers
     source          1..1055
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1..471
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="SLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTW
                     ESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPK
                     AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     CDS             472..489
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     protein_bind    539..572
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        627..970
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 agtctgattg cggcgttagc ggtagatcgc gttatcggca tggaaaacgc catgccgtgg
       61 aacctgcctg ccgatctcgc ctggtttaaa cgcaacacct taaataaacc cgtgattatg
      121 ggccgccata cctgggaatc aatcggtcgt ccgttgccag gacgcaaaaa tattatcctc
      181 agcagtcaac cgggtacgga cgatcgcgta acgtgggtga agtcggtgga tgaagccatc
      241 gcggcgtgtg gtgacgtacc agaaatcatg gtgattggcg gcggtcgcgt ttatgaacag
      301 ttcttgccaa aagcgcaaaa actgtatctg acgcatatcg acgcagaagt ggaaggcgac
      361 acccatttcc cggattacga gccggatgac tgggaatcgg tattcagcga attccacgat
      421 gctgatgcgc agaactctca cagctattgc tttgagattc tggagcggcg gcatcatcat
      481 catcatcatt aactcgagca gctgaagctt cgtacgctgc aggtcgacaa cccttaatat
      541 aacttcgtat aatgtatgct atacgaagtt attaggtcta gagatctgtt tagcttgcct
      601 cgtccccgcc gggtcacccg gccagcgaca tggaggccca gaataccctc cttgacagtc
      661 ttgacgtgcg cagctcaggg gcatgatgtg actgtcgccc gtacatttag cccatacatc
      721 cccatgtata atcatttgca tccatacatt ttgatggccg cacggcgcga agcaaaaatt
      781 acggctcctc gctgcagacc tgcgagcagg gaaacgctcc cctcacagac gcgttgaatt
      841 gtccccacgc cgcgcccctg tagagaaata taaaaggtta ggatttgcca ctgaggttct
      901 tctttcatat acttcctttt aaaatcttgc taggatacag ttctcacatc acatccgaac
      961 ataaacaacc atgggtatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga
     1021 tcccaccgcc gccttctatg aaaggttggg cttcg
//
