LOCUS       sequence-73280-e        1034 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Used as a PCR template for tagging yeast proteins with the DHFR tag 
            by homologous recombination and selection for phleomycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1034)
  AUTHORS   Hoskins AA, Friedman LJ, Gallagher SS, Crawford DJ, Anderson EG, 
            Wombacher R, Ramirez N, Cornish VW, Gelles J, Moore MJ
  TITLE     Ordered and dynamic assembly of single spliceosomes.
  JOURNAL   Science. 2011 Mar 11;331(6022):1289-95.
  PUBMED    21393538
REFERENCE   2  (bases 1 to 1034)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2011-03-11"; volume: "331(6022)"; pages: "1289-95"
COMMENT     Sequence Label: pAAH4
FEATURES             Location/Qualifiers
     source          1..1034
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             2..475
                     /codon_start=1
                     /gene="folA"
                     /product="E. coli dihydrofolate reductase"
                     /label=ecDHFR
                     /translation="SLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRHTW
                     ESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQFLPK
                     AQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
     protein_bind    540..573
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        628..971
                     /label=TEF promoter
                     /note="Ashbya gossypii TEF promoter"
ORIGIN
        1 cagtctgatt gcggcgttag cggtagatcg cgttatcggc atggaaaacg ccatgccgtg
       61 gaacctgcct gccgatctcg cctggtttaa acgcaacacc ttaaataaac ccgtgattat
      121 gggccgccat acctgggaat caatcggtcg tccgttgcca ggacgcaaaa atattatcct
      181 cagcagtcaa ccgggtacgg acgatcgcgt aacgtgggtg aagtcggtgg atgaagccat
      241 cgcggcgtgt ggtgacgtac cagaaatcat ggtgattggc ggcggtcgcg tttatgaaca
      301 gttcttgcca aaagcgcaaa aactgtatct gacgcatatc gacgcagaag tggaaggcga
      361 cacccatttc ccggattacg agccggatga ctgggaatcg gtattcagcg aattccacga
      421 tgctgatgcg cagaactctc acagctattg ctttgagatt ctggagcggc ggtaacatca
      481 tcatcatcat taactcgagc agctgaagct tcgtacgctg caggtcgaca acccttaata
      541 taacttcgta taatgtatgc tatacgaagt tattaggtct agagatctgt ttagcttgcc
      601 tcgtccccgc cgggtcaccc ggccagcgac atggaggccc agaataccct ccttgacagt
      661 cttgacgtgc gcagctcagg ggcatgatgt gactgtcgcc cgtacattta gcccatacat
      721 ccccatgtat aatcatttgc atccatacat tttgatggcc gcacggcgcg aagcaaaaat
      781 tacggctcct cgctgcagac ctgcgagcag ggaaacgctc ccctcacaga cgcgttgaat
      841 tgtccccacg ccgcgcccct gtagagaaat ataaaaggtt aggatttgcc actgaggttc
      901 ttctttcata tacttccttt taaaatcttg ctaggataca gttctcacat cacatccgaa
      961 cataaacaac catgggtatg accgaccaag cgacgcccaa cctgccatca cgagatttcg
     1021 atcccaccgc cgcc
//
