LOCUS       sequence-73282-e         892 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Pichia pastoris expression vector with AOX1 promoter and 
            N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 892)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 892)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pICXNH3
FEATURES             Location/Qualifiers
     source          1..892
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(89..95,96..113,114..119))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(144..268)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(287..307)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(311..331)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             371..400
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             416..433
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      513..759
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 tcaacggtgg tatatccagt gatttttttc tccattttag cttccttagc tcctgaaaat
       61 ctcgacggat cctaactcaa aatccacaca ttatacgagc cggaagcata aagtgtaaag
      121 cctggggtgc ctaatgcggc cgccatagtg actggatatg ttgtgtttta cagtattatg
      181 tagtctgttt tttatgcaaa atctaattta atatattgat atttatatca ttttacgttt
      241 ctcgttcagc ttttttgtac aaacttgtga tatcgaattc gctagctacc tttctcttct
      301 tttttggatc tacctttctc ttcttttttg gggatccact agttcagctc ttctgcataa
      361 taactatcta gaacaaaaac tcatctcaga agaggatctg aatagcgccg tcgaccatca
      421 tcatcatcat cattgagttt gtagccttag acatgactgt tcctcagttc aagttgggca
      481 cttacgagaa gaccggtctt gctagattct aatcaagagg atgtcagaat gccatttgcc
      541 tgagagatgc aggcttcatt tttgatactt ttttatttgt aacctatata gtataggatt
      601 ttttttgtca ttttgtttct tctcgtacga gcttgctcct gatcagccta tctcgcagct
      661 gatgaatatc ttgtggtagg ggtttgggaa aatcattcga gtttgatgtt tttcttggta
      721 tttcccactc ctcttcagag tacagaagat taagtgagac cttcgtttgt gcggatcccc
      781 cacacaccat agcttcaaaa tgtttctact ccttttttac tcttccagat tttctcggac
      841 tccgcgcatc gccgtaccac ttcaaaacac ccaagcacag catactaaat tt
//
