LOCUS       sequence-73285-e         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Pichia pastoris expression vector with AOX1 promoter and 
            N-terminal 10x His tag, GFP and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pICXNHG3
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..269)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(288..308)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             372..401
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             417..434
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      514..760
                     /gene="Pichia pastoris AOX1"
                     /label=AOX1 terminator
                     /note="transcription terminator for AOX1"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcag cttttttgta caaacttgtg atatcgaatt cgctagctac ctttctcttc
      301 ttttttggat ctacctttct cttctttttt ggggatccac tagttcagct cttctgcata
      361 ataactatct agaacaaaaa ctcatctcag aagaggatct gaatagcgcc gtcgaccatc
      421 atcatcatca tcattgagtt tgtagcctta gacatgactg ttcctcagtt caagttgggc
      481 acttacgaga agaccggtct tgctagattc taatcaagag gatgtcagaa tgccatttgc
      541 ctgagagatg caggcttcat ttttgatact tttttatttg taacctatat agtataggat
      601 tttttttgtc attttgtttc ttctcgtacg agcttgctcc tgatcagcct atctcgcagc
      661 tgatgaatat cttgtggtag gggtttggga aaatcattcg agtttgatgt ttttcttggt
      721 atttcccact cctcttcaga gtacagaaga ttaagtgaga ccttcgtttg tgcggatccc
      781 ccacacacca tagcttcaaa atgtttctac tcctttttta ctcttccaga ttttctcgga
      841 ctccgcgcat cgccgtacca cttcaaa
//
