LOCUS       sequence-73290-e         746 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Xenopus oocyte expression vector with SP6 promoter and 
            N-terminal chicken alpha7 signal sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 746)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 746)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pTLNXA7
FEATURES             Location/Qualifiers
     source          1..746
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        complement(join(90..96,97..114,115..120))
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     protein_bind    complement(145..269)
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             complement(288..308)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(312..332)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     primer_bind     546..562
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     547..563
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     3'UTR           613..738
                     /label=Xenopus globin 3'-UTR
                     /note="3'-UTR of the major beta-globin gene of Xenopus 
                     laevis"
ORIGIN
        1 atcaacggtg gtatatccag tgattttttt ctccatttta gcttccttag ctcctgaaaa
       61 tctcgacgga tcctaactca aaatccacac attatacgag ccggaagcat aaagtgtaaa
      121 gcctggggtg cctaatgcgg ccgccatagt gactggatat gttgtgtttt acagtattat
      181 gtagtctgtt ttttatgcaa aatctaattt aatatattga tatttatatc attttacgtt
      241 tctcgttcag cttttttgta caaacttgtg atatcgaatt cgctagctac ctttctcttc
      301 ttttttggat ctacctttct cttctttttt ggggatccac tagttcagct cttctgcata
      361 atccggtctg aaggaggtgg ctcgtctcgg gggctgggga tgggatggtg ggtgttggat
      421 gcatctcccg ggatttatct tgagaatata aagacccaga ggttgagata gagagaaaga
      481 aagatcgggt cgaccacgaa ttcttgctcc aggtccagac aggacgcttt agagcacaat
      541 ggatctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
      601 tggttaccac taaaccagcc tcaagaacac ccgaatggag tctctaagct acataatacc
      661 aacttacact ttacaaaatg ttgtccccca aaatgtagcc attcgtatct gctcctaata
      721 aaaagaaagt ttcttcacat tctaaa
//
