LOCUS       sequence-73291-e         993 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 993)
  AUTHORS   Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR
  TITLE     The coordinate release of cytochrome c during apoptosis is rapid, 
            complete and kinetically invariant.
  JOURNAL   Nat Cell Biol. 2000 Mar;2(3):156-62.
  PUBMED    10707086
REFERENCE   2  (bases 1 to 993)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2000-03"; volume: "2(3)"; pages: "156-62"
COMMENT     Sequence Label: pCytochrome C-GFP
FEATURES             Location/Qualifiers
     source          1..993
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      388..397
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(394..396,397..399,400..912)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(439..460)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(700..719)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggac tcagatctcg acctaatatg
       61 ggtgatgttg aaaaaggcaa gaagattttt gttcagaagt gtgcccagtg ccacactgtg
      121 gaaaagggag gcaagcataa gactggacca aatctccacg gtctgttcgg gcggaagaca
      181 ggccaggctg ctggattctc ttacacagat gccaacaaga acaaaggcat cacctgggga
      241 gaggataccc tgatggagta tttggagaat cccaaaaagt acatccctgg aacaaaaatg
      301 atcttcgctg gaattaagaa gaagggagaa agggcagacc taatagctta tcttaaaaag
      361 gctactaatg agaaagatcc accggtcgcc accatggtga gcaagggcga ggagctgttc
      421 accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca caagttcagc
      481 gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa gttcatctgc
      541 accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac ctacggcgtg
      601 cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa gtccgccatg
      661 cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa ctacaagacc
      721 cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct gaagggcatc
      781 gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta caacagccac
      841 aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt caagatccgc
      901 cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa cacccccatc
      961 ggcgacggcc ccgtgctgct gcccgacaac cac
//
