LOCUS       sequence-73295-e         909 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning mammalian cell line expression vector with CMV promoter 
            and C-terminal 3C protease cleavage site, EGFP, Myc tag and 
            streptavidin binding peptide.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 909)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 909)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pcDXC3GMS
FEATURES             Location/Qualifiers
     source          1..909
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     36..55
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        36..54
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    90..252
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             complement(304..609)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(406..425)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 actagagaac ccactgctta ctggcttatc gaaattaata cgactcacta tagggagacc
       61 caagctggct agcgtttaaa cttaagctta gcgcagaggc ttggggcagc cgagcggcag
      121 ccaggccccg gcccgggcct cggttccaga agggagagga gcccgccaag gcgcgcaaga
      181 gagcgggctg cctcgcagtc cgagccggag agggagcgcg agccgcgccg gccccggacg
      241 gcctccgaaa ccatgagtag aagagcgagc tgcagactgg ctgtgtataa gggagcctga
      301 catttatatt ccccagaaca tcaggttaat ggcgtttttg atgtcatttt cgcggtggct
      361 gagatcagcc acttcttccc cgataacgga gaccggcaca ctggccatat cggtggtcat
      421 catgcgccag ctttcatccc cgatatgcac caccgggtaa agttcacggg agactttatc
      481 tgacagcaga cgtgcactgg ccagggggat caccatccgt cgcccgggcg tgtcaataat
      541 atcactctgt acatccacaa acagacgata acggctctct cttttatagg tgtaaacctt
      601 aaactgcatt tcaccagccc ctgttctcgt cagcaaaaga gccgttcatt tcaataaacc
      661 gggcgacctc agccatccct tcctgatttt ccgctttcca gcgttcggca cgcagacgac
      721 gggcttcatt ctgcatggtt gtgcttacca gaccggagat attgacatca tatatgcctt
      781 gagcaactga tagctgtcgc tgtcaactgt cactgtaata cgctgcttca tagcatacct
      841 ctttttgaca tacttcgggt atacatatca gtatatattc ttataccgca aaaatcagcg
      901 cgcaaatac
//
