LOCUS       sequence-73296-e         994 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 994)
  AUTHORS   Goldstein JC, Waterhouse NJ, Juin P, Evan GI, Green DR
  TITLE     The coordinate release of cytochrome c during apoptosis is rapid, 
            complete and kinetically invariant.
  JOURNAL   Nat Cell Biol. 2000 Mar;2(3):156-62.
  PUBMED    10707086
REFERENCE   2  (bases 1 to 994)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Cell 
            Biol."; date: "2000-03"; volume: "2(3)"; pages: "156-62"
COMMENT     Sequence Label: pBabe(LTR)-Cytochrome C-GFP
FEATURES             Location/Qualifiers
     source          1..994
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      376..385
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(382..384,385..387,388..900)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(427..448)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(688..707)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 cagccctcac tccttctcta ggcgccggcc ggatctcgac ctaatatggg tgatgttgaa
       61 aaaggcaaga agatttttgt tcagaagtgt gcccagtgcc acactgtgga aaagggaggc
      121 aagcataaga ctggaccaaa tctccacggt ctgttcgggc ggaagacagg ccaggctgct
      181 ggattctctt acacagatgc caacaagaac aaaggcatca cctggggaga ggataccctg
      241 atggagtatt tggagaatcc caaaaagtac atccctggaa caaaaatgat cttcgctgga
      301 attaagaaga agggagaaag ggcagaccta atagcttatc ttaaaaaggc tactaatgag
      361 aaagatccac cggtcgccac catggtgagc aagggcgagg agctgttcac cggggtggtg
      421 cccatcctgg tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag
      481 ggcgagggcg atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag
      541 ctgcccgtgc cctggcccac cctcgtgacc accctgacct acggcgtgca gtgcttcagc
      601 cgctaccccg accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac
      661 gtccaggagc gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg
      721 aagttcgagg gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag
      781 gacggcaaca tcctggggca caagctggag tacaactaca acagccacaa cgtctatatc
      841 atggccgaca agcagaagaa cggcatcaag gtgaacttca agatccgcca caacatcgag
      901 gacggcagcg tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc
      961 gtgctgctgc ccgacaacca ctacctgagc accc
//
