LOCUS       sequence-73356-e         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Stanton BC, Nielsen AA, Tamsir A, Clancy K, Peterson T, Voigt CA
  TITLE     Genomic mining of prokaryotic repressors for orthogonal logic gates.
  JOURNAL   Nat Chem Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411.
  PUBMED    24316737
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2013 Dec 8. doi: 10.1038/nchembio.1411."
COMMENT     Sequence Label: pNAND-PhlF
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        join(3..8,9..24,25..31)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    37..56
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     terminator      716..787
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      803..830
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcgctc acaattctat
       61 ggactatgtt tgaaagggag aaatactaga tggcacgtac cccgagccgt agcagcattg
      121 gtagcctgcg tagtccgcat acccataaag caattctgac cagcaccatt gaaatcctga
      181 aagaatgtgg ttatagcggt ctgagcattg aaagcgttgc acgtcgtgcc ggtgcaagca
      241 aaccgaccat ttatcgttgg tggaccaata aagcagcact gattgccgaa gtgtatgaaa
      301 atgaaagcga acaggtgcgt aaatttccgg atctgggtag ctttaaagcc gatctggatt
      361 ttctgctgcg taatctgtgg aaagtttggc gtgaaaccat ttgtggtgaa gcatttcgtt
      421 gtgttattgc agaagcacag ctggaccctg caaccctgac ccagctgaaa gatcagttta
      481 tggaacgtcg tcgtgagatg ccgaaaaaac tggttgaaaa tgccattagc aatggtgaac
      541 tgccgaaaga taccaatcgt gaactgctgc tggatatgat ttttggtttt tgttggtatc
      601 gcctgctgac cgaacagctg accgttgaac aggatattga agaatttacc ttcctgctga
      661 ttaatggtgt ttgtccgggt acacagcgtt aactagggcc cataccccca ggcatcaaat
      721 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
      781 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//
