LOCUS       sequence-7336-ex        8390 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8390)
  AUTHORS   Park JY, Luo Q, Jiang W, Kang Q, Peng Y, Strom C, Luu HH, Haydon RC,
            He TC
  TITLE     Dual regulation of gene expression mediated by tetracycline and Cre 
            recombinase.
  JOURNAL   Biotechniques. 2004 Mar . 36(3):390-2, 394, 396.
  PUBMED    15038152
REFERENCE   2  (bases 1 to 8390)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biotechniques. 2004 Mar . 36(3):390-2, 394, 396."
COMMENT     Sequence Label: pTLOX
FEATURES             Location/Qualifiers
     source          1..8390
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        262..641
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        642..845
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     promoter        890..908
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     intron          948..1520
                     /label=beta-globin intron
                     /note="intron from rabbit beta-globin gene"
     primer_bind     1528..1547
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     promoter        1575..1593
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             1604..2224
                     /codon_start=1
                     /gene="tetR from transposon Tn10"
                     /product="tetracycline repressor TetR"
                     /label=TetR
                     /note="TetR binds to the tetracycline operator tetO to 
                     inhibit transcription. This inhibition can be relieved by 
                     adding tetracycline or doxycycline."
                     /translation="MSRLDKSKVINSALELLNEVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALAIEMLDRHHTHFCPLEGESWQDFLRNNAKSFRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEDQEHQVAKEERETPTT
                     DSMPPLLRQAIELFDHQGAEPAFLFGLELIICGLEKQLKCESGS"
     primer_bind     complement(1677..1696)
                     /label=Tet-R
                     /note="Tetracycline resistance gene, reverse primer"
     misc_feature    2378..2950
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    2401..2952
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2545..2562)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2772..2791
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             3019..3813
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVLRLSAQGRP
                     VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
                     SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
                     GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
                     LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(3073..3092)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     3683..3702
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     polyA_signal    3987..4121
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     CDS             4130..4171
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     enhancer        4176..4555
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        4556..4759
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     protein_bind    4761..4779
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4803..4821
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4824..4842
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    4946..4979
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     regulatory      5029..5038
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(5035..5037,5038..5040,5041..5751)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(5035..5037,5038..5040,5041..5751)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(5080..5101)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(5341..5360)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     5688..5709
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     polyA_signal    5890..6011
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     protein_bind    6037..6070
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(6149..6166)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6155..6379
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(6467..6484)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6638..7223)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6718..6737)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7394..8185,8186..8254))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     8017..8036
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8255..8359)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattaatta aggcgcgccg
      181 tttaaactaa ttgcatgaag aatctgctta gggttaggcg ttttgcgctg cttcgcgatg
      241 tacgggccag atatacgcgt tgacattgat tattgactag ttattaatag taatcaatta
      301 cggggtcatt agttcatagc ccatatatgg agttccgcgt tacataactt acggtaaatg
      361 gcccgcctgg ctgaccgccc aacgaccccc gcccattgac gtcaataatg acgtatgttc
      421 ccatagtaac gccaataggg actttccatt gacgtcaatg ggtggagtat ttacggtaaa
      481 ctgcccactt ggcagtacat caagtgtatc atatgccaag tacgccccct attgacgtca
      541 atgacggtaa atggcccgcc tggcattatg cccagtacat gaccttatgg gactttccta
      601 cttggcagta catctacgta ttagtcatcg ctattaccat ggtgatgcgg ttttggcagt
      661 acatcaatgg gcgtggatag cggtttgact cacggggatt tccaagtctc caccccattg
      721 acgtcaatgg gagtttgttt tggcaccaaa atcaacggga ctttccaaaa tgtcgtaaca
      781 actccgcccc attgacgcaa atgggcggta ggcgtgtacg gtgggaggtc tatataagca
      841 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
      901 actataggga gacccaagct ggctagatcg atcctgagaa cttcagggtg agtttgggga
      961 cccttgattg ttctttcttt ttcgctattg taaaattcat gttatatgga gggggcaaag
     1021 ttttcagggt gttgtttaga atgggaagat gtcccttgta tcaccatgga ccctcatgat
     1081 aattttgttt ctttcacttt ctactctgtt gacaaccatt gtctcctctt attttctttt
     1141 cattttctgt aactttttcg ttaaacttta gcttgcattt gtaacgaatt tttaaattca
     1201 cttttgttta tttgtcagat tgtaagtact ttctctaatc actttttttt caaggcaatc
     1261 agggtatatt atattgtact tcagcacagt tttagagaac aattgttata attaaatgat
     1321 aaggtagaat atttctgcat ataaattctg gctggcgtgg aaatattctt attggtagaa
     1381 acaactacat cctggtcatc atcctgcctt tctctttatg gttacaatga tatacactgt
     1441 ttgagatgag gataaaatac tctgagtcca aaccgggccc ctctgctaac catgttcatg
     1501 ccttcttctt tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt
     1561 ttggcaaaga attgtaatac gactcactat agggcgaatt gatatgtcta gattagataa
     1621 aagtaaagtg attaacagcg cattagagct gcttaatgag gtcggaatcg aaggtttaac
     1681 aacccgtaaa ctcgcccaga agctaggtgt agagcagcct acattgtatt ggcatgtaaa
     1741 aaataagcgg gctttgctcg acgccttagc cattgagatg ttagataggc accatactca
     1801 cttttgccct ttagaagggg aaagctggca agatttttta cgtaataacg ctaaaagttt
     1861 tagatgtgct ttactaagtc atcgcgatgg agcaaaagta catttaggta cacggcctac
     1921 agaaaaacag tatgaaactc tcgaaaatca attagccttt ttatgccaac aaggtttttc
     1981 actagagaat gcattatatg cactcagcgc tgtggggcat tttactttag gttgcgtatt
     2041 ggaagatcaa gagcatcaag tcgctaaaga agaaagggaa acacctacta ctgatagtat
     2101 gccgccatta ttacgacaag ctatcgaatt atttgatcac caaggtgcag agccagcctt
     2161 cttattcggc cttgaattga tcatatgcgg attagaaaaa caacttaaat gtgaaagtgg
     2221 gtccgcaccg ggtatggcgg cggcggttcg gatgaacatc cagatgctgc tggaggcggc
     2281 cgactatctg gagcggcggg agagagaagc tgaacatggt tatgcctcca tgttaccata
     2341 caataacaag gactaaggat cccgggaatt aattccgccc ctctccctcc ccccccccta
     2401 acgttactgg ccgaagccgc ttggaataag gccggtgtgc gtttgtctat atgttatttt
     2461 ccaccatatt gccgtctttt ggcaatgtga gggcccggaa acctggccct gtcttcttga
     2521 cgagcattcc taggggtctt tcccctctcg ccaaaggaat gcaaggtctg ttgaatgtcg
     2581 tgaaggaagc agttcctctg gaagcttctt gaagacaaac aacgtctgta gcgacccttt
     2641 gcaggcagcg gaacccccca cctggcgaca ggtgcctctg cggccaaaag ccacgtgtat
     2701 aagatacacc tgcaaaggcg gcacaacccc agtgccacgt tgtgagttgg atagttgtgg
     2761 aaagagtcaa atggctctcc tcaagcgtat tcaacaaggg gctgaaggat gcccagaagg
     2821 taccccattg tatgggatct gatctggggc ctcggtgcac atgctttaca tgtgtttagt
     2881 cgaggttaaa aaacgtctag gccccccgaa ccacggggac gtggttttcc tttgaaaaac
     2941 acgatgataa tatgnnnggg gagcttggat atccattttc ggatctgatc aagagacagg
     3001 atgaggatcg tttcgcnnat gattgaacaa gatggattgc acgcaggttc tccggccgct
     3061 tgggtggaga ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc
     3121 gccgtgttgc ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc
     3181 ggtgccctga atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc
     3241 gttccttgcg cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg
     3301 ggcgaagtgc cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc
     3361 atcatggctg atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac
     3421 caccaagcga aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat
     3481 caggatgatc tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc
     3541 aaggcgcgca tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg
     3601 aatatcatgg tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg
     3661 gcggaccgct atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc
     3721 gaatgggctg accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc
     3781 gccttctatc gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg
     3841 accaagcgac gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa
     3901 ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc
     3961 tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat
     4021 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     4081 gtttgtccaa actcatcaat gtatcttatc atgtctggat cgatccgaag gtaagcctat
     4141 ccctaaccct ctcctcggtc tcgattctac gcgttgacat tgattattga ctagttatta
     4201 atagtaatca attacggggt cattagttca tagcccatat atggagttcc gcgttacata
     4261 acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat
     4321 aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga
     4381 gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc
     4441 ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt
     4501 atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat
     4561 gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag
     4621 tctccacccc attgacgtca atgggagttt gttttggaac caaaatcaac gggactttcc
     4681 aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga
     4741 ggtctatata agcagagctc tccctatcag tgatagagat ctccctatca gtgatagagc
     4801 tctccctatc agtgatagag atctccctat cagtgataga gatcgagctg tttagtgaac
     4861 cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag acaccgaatt
     4921 ccgatcatat tcaataaccc ttaatataac ttcgtataat gtatgctata cgaagttatt
     4981 aggtctgaag aggagtttac gtccagccaa gctctagcgc taccggtcgc caccatggtg
     5041 agcaagggcg aggagctgtt caccggggtg gtgcccatcc tggtcgagct ggacggcgac
     5101 gtaaacggcc acaagttcag cgtgtccggc gagggcgagg gcgatgccac ctacggcaag
     5161 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
     5221 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
     5281 gacttcttca agtccgccat gcccgaaggc tacgtccagg agcgcaccat cttcttcaag
     5341 gacgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
     5401 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
     5461 gagtacaact acaacagcca caacgtctat atcatggccg acaagcagaa gaacggcatc
     5521 aaggtgaact tcaagatccg ccacaacatc gaggacggca gcgtgcagct cgccgaccac
     5581 taccagcaga acacccccat cggcgacggc cccgtgctgc tgcccgacaa ccactacctg
     5641 agcacccagt ccgccctgag caaagacccc aacgagaagc gcgatcacat ggtcctgctg
     5701 gagttcgtga ccgccgccgg gatcactctc ggcatggacg agctgtacaa gtccggactc
     5761 agatccaccg gatctagata actgatcata atcagccata ccacatttgt agaggtttta
     5821 cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat gaatgcaatt
     5881 gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     5941 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     6001 aatgtatctt aacgcgtgat ccggnaaccc cttaatataa cttcgtataa tgtatgctat
     6061 acgaagttat taggtccctc gaagaggttc actagctcga gccgcggagg ccttaagacc
     6121 ggtcgcggcc gctaaacccg ctgatcagcc tcgactgtgc cttctagttg ccagccatct
     6181 gttgtttgcc cctcccccgt gccttccttg accctggaag gtgccactcc cactgtcctt
     6241 tcctaataaa atgaggaaat tgcatcgcat tgtctgagta ggtgtcattc tattctgggg
     6301 ggtggggtgg ggcaggacag caagggggag gattgggaag acaatagcag gcatgctggg
     6361 gatgcggtgg gctctatggc ttctgaggcg gaaagaacca gctgcattaa tgaatcggcc
     6421 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     6481 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     6541 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     6601 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     6661 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     6721 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     6781 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
     6841 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     6901 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     6961 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     7021 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
     7081 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     7141 cttgatccgg caaacaaacc accgctggta gcggtttttt tgtttgcaag cagcagatta
     7201 cgcgcagaaa aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc
     7261 agtggaacga aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca
     7321 cctagatcct tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa
     7381 cttggtctga cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat
     7441 ttcgttcatc catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct
     7501 taccatctgg ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt
     7561 tatcagcaat aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat
     7621 ccgcctccat ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta
     7681 atagtttgcg caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg
     7741 gtatggcttc attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt
     7801 tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg
     7861 cagtgttatc actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg
     7921 taagatgctt ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc
     7981 ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa
     8041 ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac
     8101 cgctgttgag atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt
     8161 ttactttcac cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg
     8221 gaataagggc gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa
     8281 gcatttatca gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata
     8341 aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
