LOCUS       sequence-73367-e         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning mammalian cell line expression vector with CMV promoter 
            and N-terminal streptavidin binding peptide, Myc tag and 3C protease
            cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pcDXNSM3
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    89..251
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             264..377
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             378..407
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             414..437
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(486..791)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(588..607)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagctggcta gcgtttaaac ttaagcttag cgcagaggct tggggcagcc gagcggcagc
      121 caggccccgg cccgggcctc ggttccagaa gggagaggag cccgccaagg cgcgcaagag
      181 agcgggctgc ctcgcagtcc gagccggaga gggagcgcga gccgcgccgg ccccggacgg
      241 cctccgaaac catgggggcc agcatggacg agaagaccac tggttggcga ggtggacacg
      301 ttgttgaagg actggctggg gaacttgaac aacttcgtgc acgactggag catcacccac
      361 aaggtcaacg tgaaccagaa caaaaactca tctcagaaga ggatctgggt accttagaag
      421 ttttgtttca aggtccaagt agaagagcga gctgcagact ggctgtgtat aagggagcct
      481 gacatttata ttccccagaa catcaggtta atggcgtttt tgatgtcatt ttcgcggtgg
      541 ctgagatcag ccacttcttc cccgataacg gagaccggca cactggccat atcggtggtc
      601 atcatgcgcc agctttcatc cccgatatgc accaccgggt aaagttcacg ggagacttta
      661 tctgacagca gacgtgcact ggccaggggg atcaccatcc gtcgcccggg cgtgtcaata
      721 atatcactct gtacatccac aaacagacga taacggctct ctcttttata ggtgtaaacc
      781 ttaaactgca tttcaccagc ccctgttctc gtcagcaaaa gagccgttca tttcaataaa
      841 ccgggcgacc tcagccatcc cttcctgatt ttccgctttc cagcgttcgg cacgcagacg
      901 acgggcttca ttctgcatgg ttgtgcttac cagaccggag atattgacat catatatgcc
      961 ttgagcaact gatagctgtc
//
