LOCUS       sequence-73368-e         927 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning mammalian cell line expression vector with CMV promoter 
            and N-terminal streptavidin binding peptide, Myc tag, EGFP and 3C 
            protease cleavage site .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 927)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 927)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pcDXNSMG3
FEATURES             Location/Qualifiers
     source          1..927
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     30..49
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        30..48
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    84..246
                     /label=QBI SP163
                     /note="strong translational enhancer derived from the 5' 
                     UTR of the mouse VEGF gene"
     CDS             259..372
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             373..402
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             join(409..411,412..414,415..927)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(454..475)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(715..734)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaacccactg cttactggct tatcgaaatt aatacgactc actataggga gacccaagct
       61 ggctagcgtt taaacttaag cttagcgcag aggcttgggg cagccgagcg gcagccaggc
      121 cccggcccgg gcctcggttc cagaagggag aggagcccgc caaggcgcgc aagagagcgg
      181 gctgcctcgc agtccgagcc ggagagggag cgcgagccgc gccggccccg gacggcctcc
      241 gaaaccatgg gggccagcat ggacgagaag accactggtt ggcgaggtgg acacgttgtt
      301 gaaggactgg ctggggaact tgaacaactt cgtgcacgac tggagcatca cccacaaggt
      361 caacgtgaac cagaacaaaa actcatctca gaagaggatc tgggtaccat ggtgagcaag
      421 ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      481 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagctgacc
      541 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct cgtgaccacc
      601 ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca gcacgacttc
      661 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      721 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      781 gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      841 aactacaaca gccacaacgt ctatatcatg gccgacaagc agaagaacgg catcaaggtg
      901 aacttcaaga tccgccacaa catcgag
//
