LOCUS       sequence-73370-e         876 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Saccharomyces cerevisiae expression vector with GAL1 
            promoter and C-terminal 3C protease cleavage site and 10x His tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 876)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 876)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pYEXC3H
FEATURES             Location/Qualifiers
     source          1..876
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(137..156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(138..156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     promoter        complement(188..629)
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     primer_bind     complement(194..217)
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     protein_bind    512..629
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     complement(738..757)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tctcagccac cgcgaaaatg acatcaaaaa cgccattaac ctgatgttct ggggaatata
       61 aatgtcaggc tcccttatac acagccagtc tgcaggtcgc tcttcaactc atggttaagg
      121 taccaagctt aatattccct atagtgagtc gtattacagc tgctagtagt ccgatccggg
      181 gttttttctc cttgacgtta aagtatagag gtatattaac aattttttgt tgatactttt
      241 attacatttg aataagaagt aatacaaacc gaaaatgttg aaagtattag ttaaagtggt
      301 tatgcagttt ttgcatttat atatctgtta atagatcaaa aatcatcgct tcgctgatta
      361 attaccccag aaataaggct aaaaaactaa tcgcattatc atcctatggt tgttaatttg
      421 attcgttcat ttgaaggttt gtggggccag gttactgcca atttttcctc ttcataacca
      481 taaaagctag tattgtagaa tctttattgt tcggagcagt gcggcgcgag gcacatctgc
      541 gtttcaggaa cgcgaccggt gaagacgagg acgcacggag gagagtcttc cttcggaggg
      601 ctgtcacccg ctcggcggct tctaatccgt ggactagtgg atcgatcccc acgcgccctg
      661 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
      721 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
      781 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
      841 gcacctcgac cccaaaaaac ttgattaggg tgatgg
//
