LOCUS       sequence-73373-e         928 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning Pichia pastoris expression vector with AOX1 promoter and 
            N-terminal 10x His tag and 3C protease cleavage site.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 928)
  AUTHORS   Geertsma ER, Dutzler R
  TITLE     A versatile and efficient high-throughput cloning tool for 
            structural biology.
  JOURNAL   Biochemistry. 2011 Apr 19;50(15):3272-8. Epub 2011 Mar 25.
  PUBMED    21410291
REFERENCE   2  (bases 1 to 928)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Biochemistry."; date: "2011-04-19"; volume: "50(15)"; pages: 
            "3272-8. Epub 2011 Mar 25"
COMMENT     Sequence Label: pICXNH3
FEATURES             Location/Qualifiers
     source          1..928
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(119..142)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(143..172)
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
ORIGIN
        1 agtggctgat ctcagccacc gcgaaaatga catcaaaaac gccattaacc tgatgttctg
       61 gggaatataa atgtcaggct cccttataca cagccagtct gcagctcgct cttctacttg
      121 gaccttgaaa caaaacttct aaatgatgat gatgatggtg atgatgatgg tgcatggtta
      181 atggtaccga tccgagacgg ccggctgggc cacgtgaatt cctcgtttcg aataattagt
      241 tgttttttga tcttctcaag ttgtcgttaa aagtcgttaa aatcaaaagc ttgtcaattg
      301 gaaccagtcg caattatgaa agtaagctaa taatgatgat aaaaaaaaag gtttaagaca
      361 gggcagcttc cttctgttta tatattgctg tcaagtaggg gttagaacag ttaaattttg
      421 atcatgaacg ttaggctatc agcagtattc ccaccagaat cttggaagca tacaatgtgg
      481 agacaatgca taatcatcca aaaagcgggt gtttccccat ttgcgtttcg gcacaggtgc
      541 accggggttc agaagcgata gagagactgc gctaagcatt aatgagatta tttttgagca
      601 ttcgtcaatc aataccaaac aagacaaacg gtatgccgac ttttggaagt ttctttttga
      661 ccaactggcc gttagcattt caacgaacca aacttagttc atcttggatg agatcacgct
      721 tttgtcatat taggttccaa gacagcgttt aaactgtcag ttttgggcca tttggggaac
      781 atgaaactat ttgaccccac actcagaaag ccctcatctg gagtgatgtt cgggtgtaat
      841 gcggagcttg ttgcattcgg aaataaacaa acatgaacct cgccaggggg gccaggatag
      901 acaggctaat aaagtcatgg tgttagta
//
