LOCUS       sequence-7338-ex         959 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 959)
  AUTHORS   Luu HH, Zhou L, Haydon RC, Deyrup AT, Montag AG, Huo D, Heck R, 
            Heizmann CW, Peabody TD, Simon MA, He TC
  TITLE     Increased expression of S100A6 is associated with decreased 
            metastasis and inhibition of cell migration and anchorage 
            independent growth in human osteosarcoma.
  JOURNAL   Cancer Lett. 2005 Nov 8. 229(1):135-48.
  PUBMED    16157226
REFERENCE   2  (bases 1 to 959)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cancer 
            Lett. 2005 Nov 8. 229(1):135-48."
COMMENT     Sequence Label: pHAHA
FEATURES             Location/Qualifiers
     source          1..959
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        384..587
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     538..558
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     584..608
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             659..685
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     polyA_signal    714..848
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(738..757)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     792..811
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gttgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggagt atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtccgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttac gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atggtgatgc ggttttggca gtacaccaat gggcgtggat
      421 agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat gggagtttgt
      481 tttggcacca aaatcaacgg gactttccaa aatgtcgtaa taaccccgcc ccgttgacgc
      541 aaatgggcgg taggcgtgta cggtgggagg tctatataag cagagctcgt ttagtgaacc
      601 gtcagatctc tagaagctgg gtaccgaaat gggatacgat gttccagatt acgcttcgta
      661 cgatgttcca gattacgcaa gcttgctagc ggccgctgag gccggcaagg ccggatccag
      721 acatgataag atacattgat gagtttggac aaaccacaac tagaatgcag tgaaaaaaat
      781 gctttatttg tgaaatttgt gatgctattg ctttatttgt aaccattata agctgcaata
      841 aacaagttaa caacaacaat tgcattcatt ttatgtttca ggttcagggg gaggtgtggg
      901 aggtttttta aagcaagtaa aacctctaca aatgtggtat ggctgattat gatccggct
//
