LOCUS       sequence-73382-e        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cas9 from S. pyogenes with 2A-EGFP, and cloning backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Ran FA, Hsu PD, Wright J, Agarwala V, Scott DA, Zhang F
  TITLE     Genome engineering using the CRISPR-Cas9 system.
  JOURNAL   Nat Protoc. 2013 Nov;8(11):2281-308. doi: 10.1038/nprot.2013.143. 
            Epub 2013 Oct 24.
  PUBMED    24157548
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nprot.2013.143"; journalName: "Nat Protoc"; date: 
            "2013-11"; volume: "8"; issue: "11"; pages: "2281-308"
COMMENT     Sequence Label: pSpCas9(BB)-2A-GFP (PX458)
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(30..83)
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             complement(99..146)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ccggtgaaca gctcctcgcc cttgctcact gggccaggat tctcctcgac gtcaccgcat
       61 gttagcagac ttcctctgcc ctctccactg ccgaattcct ttttcttttt tgcctggccg
      121 gcctttttcg tggccgccgg ccttttgtcg cctcccagct gagacaggtc gatccgtgtc
      181 tcgtacaggc cggtgatgct ctggtggatc agggtggcgt ccagcacctc tttggtgctg
      241 gtgtacctct tccggtcgat ggtggtgtca aagtacttga aggcggcagg ggctcccaga
      301 ttggtcaggg taaacaggtg gatgatattc tcggcctgct ctctgatggg cttatcccgg
      361 tgcttgttgt aggcggacag cactttgtcc agattagcgt cggccaggat cactctcttg
      421 gagaactcgc tgatctgctc gatgatctcg tccaggtagt gcttgtgctg ttccacaaac
      481 agctgtttct gctcattatc ctcgggggag cccttcagct tctcatagtg gctggccagg
      541 tacaggaagt tcacatattt ggagggcagg gccagttcgt ttcccttctg cagttcgccg
      601 gcagaggcca gcattctctt ccggccgttt tccagctcga acagggagta cttaggcagc
      661 ttgatgatca ggtccttttt cacttctttg tagcccttgg cttccagaaa gtcgatggga
      721 ttcttctcga agctgcttct ttccatgatg gtgatcccca gcagctcttt cacactcttc
      781 agtttcttgg acttgccctt ttccactttg gccaccacca gcacagaata ggccacggtg
      841 gggctgtcga agccgccgta cttcttaggg tcccagtcct tctttctggc gatcagctta
      901 tcgctgttcc tcttgggcag gatagactct ttgctgaagc cgcctgtctg cacctcggtc
      961 tttttcacga tattcacttg gggcatgctc agcactttcc gcacggtggc aaaatcccgg
     1021 cccttatcc
//
