LOCUS       sequence-73383-e         989 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FX cloning insect cell expression vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 989)
  AUTHORS   Weinert C, Morger D, Djekic A, Grutter MG, Mittl PR
  TITLE     Crystal structure of TRIM20 C-terminal coiled-coil/B30.2 fragment: 
            implications for the recognition of higher order oligomers.
  JOURNAL   Sci Rep. 2015 Jun 4;5:10819. doi: 10.1038/srep10819.
  PUBMED    26043233
REFERENCE   2  (bases 1 to 989)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2015-06-4"; volume: "5:10819. doi"; pages: " 
            10.1038/srep10819"
COMMENT     Sequence Label: pFBXNH3
FEATURES             Location/Qualifiers
     source          1..989
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..135
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             136..159
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     CDS             complement(208..513)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(310..329)
                     /label=ccdB-fwd
                     /note="ccdB gene, forward primer"
ORIGIN
        1 aaaaacctat aaatattccg gattattcat accgtcccac catcgggcgc ggatcccggt
       61 ccgaagcgcg cggaattcaa aggcctacgt cgacgagctc atatgcacca tcatcatcac
      121 catcatcatc atcatttaga agttttgttt caaggtccaa gtagaagagc gagctgcaga
      181 ctggctgtgt ataagggagc ctgacattta tattccccag aacatcaggt taatggcgtt
      241 tttgatgtca ttttcgcggt ggctgagatc agccacttct tccccgataa cggagaccgg
      301 cacactggcc atatcggtgg tcatcatgcg ccagctttca tccccgatat gcaccaccgg
      361 gtaaagttca cgggagactt tatctgacag cagacgtgca ctggccaggg ggatcaccat
      421 ccgtcgcccg ggcgtgtcaa taatatcact ctgtacatcc acaaacagac gataacggct
      481 ctctctttta taggtgtaaa ccttaaactg catttcacca gcccctgttc tcgtcagcaa
      541 aagagccgtt catttcaata aaccgggcga cctcagccat cccttcctga ttttccgctt
      601 tccagcgttc ggcacgcaga cgacgggctt cattctgcat ggttgtgctt accagaccgg
      661 agatattgac atcatatatg ccttgagcaa ctgatagctg tcgctgtcaa ctgtcactgt
      721 aatacgctgc ttcatagcat acctcttttt gacatacttc gggtatacat atcagtatat
      781 attcttatac cgcaaaaatc agcgcgcaaa tacgcatact gttatctggc ttttagtaag
      841 ccggatccac gcgtttacgc cccgccctgc cactcatcgc agtactgttg taattcatta
      901 agcattctgc cgacatggaa gccatcacaa acggcatgat gaacctgaat cgccagcggc
      961 atcagcacct tgtcgccttg cgtataata
//
