LOCUS       sequence-73398-e        1019 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1019)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1019)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS1.1 (JW493)
FEATURES             Location/Qualifiers
     source          1..1019
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(129..253)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      260..901
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(923..943)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(923..941)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(962..978)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(962..978)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(975..997)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    986..1002
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 ctccgttatc ggggaagaag tggctgatct cagccaccgc gaaaatgaca tcaaaaacgc
       61 cattaacctg atgttctggg gaatataaat gtcaggctcc cttatacaca gccagtctgc
      121 acctcgacca tagtgactgg atatgttgtg ttttacagta ttatgtagtc tgttttttat
      181 gcaaaatcta atttaatata ttgatattta tatcatttta cgtttctcgt tcagctttct
      241 tgtacaaagt ggtgatccta gctttcgttc gtatcatcgg tttcgacaac gttcgtcaag
      301 ttcaatgcat cagtttcatt gcgcacacac cagaatccta ctgagtttga gtattatggc
      361 attgggaaaa ctgtttttct tgtaccattt gttgtgcttg taatttactg tgttttttat
      421 tcggttttcg ctatcgaact gtgaaatgga aatggatgga gaagagttaa tgaatgatat
      481 ggtccttttg ttcattctca aattaatatt atttgttttt tctcttattt gttgtgtgtt
      541 gaatttgaaa ttataagaga tatgcaaaca ttttgttttg agtaaaaatg tgtcaaatcg
      601 tggcctctaa tgaccgaagt taatatgagg agtaaaacac ttgtagttgt accattatgc
      661 ttattcacta ggcaacaaat atattttcag acctagaaaa gctgcaaatg ttactgaata
      721 caagtatgtc ctcttgtgtt ttagacattt atgaactttc ctttatgtaa ttttccagaa
      781 tccttgtcag attctaatca ttgctttata attatagtta tactcatgga tttgtagttg
      841 agtatgaaaa tattttttaa tgcattttat gacttgccaa ttgattgaca acatgcatca
      901 atcggagctc cagcttttgt tccctttagt gagggttaat tgcgcgcttg gcgtaatcat
      961 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacga
//
