LOCUS       sequence-73400-e        1081 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1081)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1081)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS1.1 (JW493)
FEATURES             Location/Qualifiers
     source          1..1081
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..26
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        7..25
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     50..66
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     51..67
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        604..949
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     primer_bind     892..912
                     /label=35S promoter
                     /note="CaMV 35S promoter, forward primer"
     protein_bind    996..1020
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 cgcgcgtaat acgactcact atagggcgaa ttgggtaccg ggccccccct cgaggtcgac
       61 ggtatcgata agcttgatat cgaattcctg cagcccgggg gatccattcg gtccccagat
      121 tagccttttc aatttcagaa agaatgctaa cccacagatg gttagagagg cttacgcagc
      181 aggtctcatc aagacgatct acccgagcaa taatctccag gaaatcaaat accttcccaa
      241 gaaggttaaa gatgcagtca aaagattcag gactaactgc atcaagaaca cagagaaaga
      301 tatatttctc aagatcagaa gtactattcc agtatggacg attcaaggct tgcttcacaa
      361 accaaggcaa gtaatagaga ttggagtctc taaaaaggta gttcccactg aatcaaaggc
      421 catggagtca aagattcaaa tagaggacct aacagaactc gccgtaaaga ctggcgaaca
      481 gttcatacag agtctcttac gactcaatga caagaagaaa atcttcgtca acatggtgga
      541 gcacgacaca cttgtctact ccaaaaatat caaagataca gtctcagaag accaaagggc
      601 aattgagact tttcaacaaa gggtaatatc cggaaacctc ctcggattcc attgcccagc
      661 tatctgtcac tttattgtga agatagtgga aaaggaaggt ggctcctaca aatgccatca
      721 ttgcgataaa ggaaaggcca tcgttgaaga tgcctctgcc gacagtggtc ccaaagatgg
      781 acccccaccc acgaggagca tcgtggaaaa agaagacgtt ccaaccacgt cttcaaagca
      841 agtggattga tgtgatatct ccactgacgt aagggatgac gcacaatccc actatccttc
      901 gcaagaccct tcctctatat aaggaagttc atttcatttg gagagaacac gggggacgag
      961 ctgtgatttt tctctacaag cgaatctaga ggatcacaag tttgtacaaa aaagctgaac
     1021 gagaaacgta aaatgatata aatatcaata tattaaatta gattttgcat aaaaaacaga
     1081 c
//
