LOCUS       sequence-73411-e         948 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 948)
  AUTHORS   Liu J, Lou Y, Yokota H, Adams PD, Kim R, Kim SH
  TITLE     Crystal structures of an NAD kinase from Archaeoglobus fulgidus in 
            complex with ATP, NAD, or NADP.
  JOURNAL   J Mol Biol. 2005 Nov 25. 354(2):289-303.
  PUBMED    16242716
REFERENCE   2  (bases 1 to 948)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2005 Nov 25. 354(2):289-303."
COMMENT     Sequence Label: pLIC.B3.AF2373
FEATURES             Location/Qualifiers
     source          1..948
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             35..52
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             74..94
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     primer_bind     complement(890..918)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
ORIGIN
        1 actttaagaa ggagatatac atatgggcag cagacatcat catcatcatc acgattacga
       61 tatcccaacg accgaaaacc tttacttcca gggcggcggt ggtggcggca tgagggccgc
      121 agtggtttac aaaaccgatg gtcacgtgaa gaggattgag gaagctttga aaaggctgga
      181 ggttgaggtt gagctcttca accagccttc agaagagctt gagaattttg acttcattgt
      241 gagtgttggg ggagacggga caattctgag aatactgcag aagctgaagc gctgcccgcc
      301 cattttcggg ataaacaccg gaagagtcgg tctgcttacc cacgccagcc ctgaaaactt
      361 tgaggttgag cttaaaaagg cggtggagaa gtttgaggtt gagaggtttc cgagagtaag
      421 ctgctccgca atgcccgatg ttcttgccct caacgagatt gccgttttga gcagaaaacc
      481 ggcgaagatg atagacgtcg ctttgagggt cgatggcgtg gaggtggaca gaataaggtg
      541 cgacggcttt attgttgcaa ctcagattgg ctccacaggc tacgccttct ctgctggagg
      601 gccggtagtt gagccctacc tcgaatgctt catcctcatc cccattgcac ccttccgctt
      661 cggctggaag ccctacgtgg tcagcatgga gaggaaaatt gaggttattg ctgagaaagc
      721 cattgtggtg gctgatgggc agaagagcgt ggattttgat ggtgagataa ccatagaaaa
      781 gtcggaattt ccggctgtgt tcttcaaaaa cgagaaaagg ttcaggaacc ttttcgggaa
      841 agttaggagc ataggttagg ggcgcaaagg agaagaactg ttcactggtg ttgtcccaat
      901 tcttgttgaa ttagatggtg atgtttaatg ggcacaaatt ttctgtca
//
