LOCUS       sequence-73419-e         615 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for integrative inducible expression of mycobacterial proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 615)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 615)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-KiT
FEATURES             Location/Qualifiers
     source          1..615
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     23..42
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(192..238)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      267..309
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(328..351)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(424..441)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 agcaagacgt ttcccgttga atatggctca taacacccct tgtattactg tttatgtaag
       61 cagacagttt tattgttcat gatgatatat ttttatcttg tgcaatgtaa catcagagat
      121 tttgagacac aacgtggctt tccccccccc ccgacgtcag gtggctagct gatcaccgcg
      181 gccatgatgg cataaaacga aaggcccagt ctttcgactg agcctttcgt tttatttgat
      241 gcctggcagt cgatcgtacg ctagttataa aacgaaaggc ccagtctttc gactgagcct
      301 ttcgttttag ttaactgata gcttttactt gtcgtcgtcg tccttgtagt caagcttaat
      361 taacacgtgc tgcaggaatt cgggccccag ctgtgcggcc gctctagaga tatcggatcc
      421 catatggtgg tggtggtggt gcattgcgaa gtgattcctc cgcatgcgga tcgtgctcat
      481 ttcgggcggc gaatctctcg gcgtcgaaat gatgtatgcc gtgctggtcg tacacacggg
      541 tactcccaga gcctatctat cactgatagg gaggacaaac tctatcactg atagggagtt
      601 ctcccgctcg tcaga
//
