LOCUS       sequence-73420-e         626 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for integrative constitutive expression of mycobacterial proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 626)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 626)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-Ki
FEATURES             Location/Qualifiers
     source          1..626
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     34..53
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(203..249)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      278..320
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(339..362)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(435..452)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 tcgcggcctc gagcaagacg tttcccgttg aatatggctc ataacacccc ttgtattact
       61 gtttatgtaa gcagacagtt ttattgttca tgatgatata tttttatctt gtgcaatgta
      121 acatcagaga ttttgagaca caacgtggct ttcccccccc cccgacgtca ggtggctagc
      181 tgatcaccgc ggccatgatg gcataaaacg aaaggcccag tctttcgact gagcctttcg
      241 ttttatttga tgcctggcag tcgatcgtac gctagttata aaacgaaagg cccagtcttt
      301 cgactgagcc tttcgtttta gttaactgat agcttttact tgtcgtcgtc gtccttgtag
      361 tcaagcttaa ttaacacgtg ctgcaggaat tcgggcccca gctgtgcggc cgctctagag
      421 atatcggatc ccatatggtg gtggtggtgg tgcattgcga agtgattcct ccgcatgcgg
      481 atcgtgctca tttcgggcgg cgaatctctc ggcgtcgaaa tgatgtatgc cgtgctggtc
      541 gtacacacgg gtactcccag agcctatcta tcactgatag ggaggacaaa ctctatcact
      601 gatagggagt tctcccgctc gtcaga
//
