LOCUS       sequence-73421-e         574 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for ectopic constitutive expression of two mycobacterial proteins in
            one vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 574)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 574)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-2K
FEATURES             Location/Qualifiers
     source          1..574
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(204..250)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      279..321
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(340..363)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atcgcggcct cgagcaagac gtttcccgtt gaatatggct cataacaccc cttgtattac
       61 tgtttatgta agcagacagt tttattgttc atgatgatat atttttatct tgtgcaatgt
      121 aacatcagag attttgagac acaacgtggc tttccccccc ccccgacgtc aggtggctag
      181 ctgatcaccg cggccatgat ggcataaaac gaaaggccca gtctttcgac tgagcctttc
      241 gttttatttg atgcctggca gtcgatcgta cgctagttat aaaacgaaag gcccagtctt
      301 tcgactgagc ctttcgtttt agttaactga tagcttttac ttgtcgtcgt cgtccttgta
      361 gtcaagctta attaacacgt gctgcaggaa ttccattgcg aagtgattcc tccgggcccg
      421 catgcggatc gtgctcattt cgggcggcga atctctcggc gtcgaaatga tgtatgccgt
      481 gctggtcgta cacacgggta ctcccagagc ctactcgacc gagcacacgc aggacaaact
      541 cgcccacccc gggtgggttc tcccgctcgt caga
//
