LOCUS       sequence-73422-e         559 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  for ectopic hypoxia inducible expression of mycobacterial proteins.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 559)
  AUTHORS   Parikh A, Kumar D, Chawla Y, Kurthkoti K, Khan S, Varshney U, 
            Nandicoori VK
  TITLE     Development of a new generation of vectors for gene expression, gene
            replacement, and protein-protein interaction studies in 
            mycobacteria.
  JOURNAL   Appl Environ Microbiol. 2013 Mar;79(5):1718-29. doi: 
            10.1128/AEM.03695-12. Epub 2013 Jan 11.
  PUBMED    23315736
REFERENCE   2  (bases 1 to 559)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/AEM.03695-12"; journalName: "Appl Environ Microbiol"; date:
            "2013-03"; volume: "79"; issue: "5"; pages: "1718-29"
COMMENT     Sequence Label: pST-KNarK2
FEATURES             Location/Qualifiers
     source          1..559
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     40..59
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     terminator      complement(209..255)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      284..326
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(345..368)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(441..458)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 atttaatcgc ggcctcgagc aagacgtttc ccgttgaata tggctcataa caccccttgt
       61 attactgttt atgtaagcag acagttttat tgttcatgat gatatatttt tatcttgtgc
      121 aatgtaacat cagagatttt gagacacaac gtggctttcc cccccccccg acgtcaggtg
      181 gctagctgat caccgcggcc atgatggcat aaaacgaaag gcccagtctt tcgactgagc
      241 ctttcgtttt atttgatgcc tggcagtcga tcgtacgcta gttataaaac gaaaggccca
      301 gtctttcgac tgagcctttc gttttagtta actgatagct tttacttgtc gtcgtcgtcc
      361 ttgtagtcaa gcttaattaa cacgtgctgc aggaattcgg gccccagctg tgcggccgct
      421 ctagagatat cggatcccat atggtggtgg tggtggtgca ttgcgaagtg attcctccgc
      481 atgcgcgaca cgatccgggg tctcggactc cggcgaactc cgcgccccgc ccggacagcc
      541 atgcgctaac cctggcttc
//
