LOCUS       sequence-73426-e         853 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 853)
  TITLE     Integrated Genomics
REFERENCE   2  (bases 1 to 853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pACT2.2gtwy
FEATURES             Location/Qualifiers
     source          1..853
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             164..184
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             200..541
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
     CDS             554..580
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             560..586
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     protein_bind    617..741
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(766..771,772..789,790..796)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 agtataaata gacctgcaat tattaatctt ttgtttcctc gtcattgttc tcgttccctt
       61 tcttccttgt ttctttttct gcacaatatt tcaagctata ccaagcatac aatcaactcc
      121 aagctttgca aagatggata aagcggaatt aattcccgag cctccaaaaa agaagagaaa
      181 ggtcgaattg ggtaccgccg ccaattttaa tcaaagtggg aatattgctg atagctcatt
      241 gtccttcact ttcactaaca gtagcaacgg tccgaacctc ataacaactc aaacaaattc
      301 tcaagcgctt tcacaaccaa ttgcctcctc taacgttcat gataacttca tgaataatga
      361 aatcacggct agtaaaattg atgatggtaa taattcaaaa ccactgtcac ctggttggac
      421 ggaccaaact gcgtataacg cgtttggaat cactacaggg atgtttaata ccactacaat
      481 ggatgatgta tataactatc tattcgatga tgaagatacc ccaccaaacc caaaaaaaga
      541 gatctctatg gcttacccat acgatgttcc agattacgct agcttgggtg gtcatatggc
      601 catggaggcc ccatcaacaa gtttgtacaa aaaagctgaa cgagaaacgt aaaatgatat
      661 aaatatcaat atattaaatt agattttgca taaaaaacag actacataat actgtaaaac
      721 acaacatatc cagtcactat ggcggccgca ttaggcaccc caggctttac actttatgct
      781 tccggctcgt ataatgtgtg gattttgagt taggatccgt cgagattttc aggagctaag
      841 gaagctaaaa tgg
//
