LOCUS       sequence-73428-e        1023 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1023)
  AUTHORS   de Felippes FF, Wang JW, Weigel D
  TITLE     MIGS: MiRNA Induced Gene Silencing.
  JOURNAL   Plant J. 2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x.
  PUBMED    22211571
REFERENCE   2  (bases 1 to 1023)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant J. 
            2011 Dec 28. doi: 10.1111/j.1365-313X.2011.04896.x."
COMMENT     Sequence Label: MIGS1.2 (JW640)
FEATURES             Location/Qualifiers
     source          1..1023
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(133..257)
                     /gene="mutant version of attR"
                     /label=attR2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     terminator      264..905
                     /label=E9 terminator
                     /note="terminator and polyadenylation signal from the pea 
                     rbcS-E9 gene"
     primer_bind     complement(927..947)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(927..945)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(966..982)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(966..982)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(979..1001)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    990..1006
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
ORIGIN
        1 cggtctccgt tatcggggaa gaagtggctg atctcagcca ccgcgaaaat gacatcaaaa
       61 acgccattaa cctgatgttc tggggaatat aaatgtcagg ctcccttata cacagccagt
      121 ctgcacctcg accatagtga ctggatatgt tgtgttttac agtattatgt agtctgtttt
      181 ttatgcaaaa tctaatttaa tatattgata tttatatcat tttacgtttc tcgttcagct
      241 ttcttgtaca aagtggtgat cctagctttc gttcgtatca tcggtttcga caacgttcgt
      301 caagttcaat gcatcagttt cattgcgcac acaccagaat cctactgagt ttgagtatta
      361 tggcattggg aaaactgttt ttcttgtacc atttgttgtg cttgtaattt actgtgtttt
      421 ttattcggtt ttcgctatcg aactgtgaaa tggaaatgga tggagaagag ttaatgaatg
      481 atatggtcct tttgttcatt ctcaaattaa tattatttgt tttttctctt atttgttgtg
      541 tgttgaattt gaaattataa gagatatgca aacattttgt tttgagtaaa aatgtgtcaa
      601 atcgtggcct ctaatgaccg aagttaatat gaggagtaaa acacttgtag ttgtaccatt
      661 atgcttattc actaggcaac aaatatattt tcagacctag aaaagctgca aatgttactg
      721 aatacaagta tgtcctcttg tgttttagac atttatgaac tttcctttat gtaattttcc
      781 agaatccttg tcagattcta atcattgctt tataattata gttatactca tggatttgta
      841 gttgagtatg aaaatatttt ttaatgcatt ttatgacttg ccaattgatt gacaacatgc
      901 atcaatcgga gctccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
      961 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     1021 cga
//
